RT Journal Article
T1 Detection of specific immune response in sole (Solea senegalensis) against Photobacterium damselae subsp. piscicida antigens.
A1 Medina, Alberto
A1 García-Millán, Irene
A1 Martínez-Manzanares, Eduardo
A1 Moriñigo-Gutiérrez, Miguel Ángel
A1 Arijo-Andrade, Salvador
K1 Peces - Inmunología
K1 Lenguados - Inmunología
AB The pathogenic bacteria Photobacterium damselae subsp. piscicida affects the development of Solea senegalensis culture. Vaccines made with inactivated cells have produced a relative protection against the sickness, however the administration of subcellular and purified antigens as vaccine could increase the effectiveness of the immune response.Thus, the aim of this work was the determination of antigens of P. damselae subsp. piscicida involved in the specific immune response of S. senegalensis.Fish were immunized by intraperitoneal injection (i.p.) with inactivated extracellular polymeric substances (ECP) and whole cells of P. damselae subsp. piscicida, and Freund's incomplete adjuvant. Two months later fish were boosted with the same antigens. Serum from fish was collected to determine by ELISA the title of antibodies against subcellular fractions of bacteria (ECP, capsule, outer membrane proteins, O antigen and formalized whole cells). Significant differences were found between control and immunized fish, but differences between first immunization and booster were only found for O antigen and capsule.Western blots derived from 2D-PAGE of ECP and Outer Membrane Proteins (OMP), using sole immunized serum, detected two high reactive antigens from ECP. Proteins were identified, by mass spectrometry, as ATP-dependent metalloprotease and Telurite resistance proteins. In the case of OMP, three antigenic proteins were detected and identified as Nrfa Y218f, Anti-oxidant AhpC/TSA, and a protein domain DNA binding heat shock related.
PB Elsevier
YR 2018
FD 2018-12-24
LK https://hdl.handle.net/10630/29966
UL https://hdl.handle.net/10630/29966
LA eng
NO A. Medina, I. García-Millán, E. Martínez-Manazanares, M.A. Moriñigo, S. Arijo (2019). Detection of specific immune response in sole (Solea senegalensis) against Photobacterium damselae subsp. piscicida antigens. Fish and Shellfish Immunology 86: 942-946https://doi.org/10.1016/j.fsi.2018.12.050
NO Política de acceso abierto tomada de: https://v2.sherpa.ac.uk/id/publication/11293
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026