RT Journal Article
T1 In vitro rescue of immature avocado (Persea americana Mill.) embryos
T2 Rescate in vitro de embriones inmaduros de aguacate (Persea americana Mill.)
A1 Sánchez-Romero, Carolina
A1 Perán-Quesada, Rosa Amelia
A1 Márquez-Martín, Belén
A1 Barcelo-Muñoz, Araceli
A1 Pliego-Alfaro, Fernando
K1 Aguacates
K1 Plantas - Multiplicación in vitro
AB An in vitro culture protocol was developed as a means of avocado embryo rescue. Different factors including presence of cotyledons, medium texture and cold or gibberellic acid pretreatments, were studied. To better understand the germination process in this recalcitrant species, immature zygotic embryos at different stages were used in these experiments. Optimum results were dependant on the embryo developmental stage. Whereas smaller embryos (5 mm long) germinated better in M1 liquid medium, 15 mm long embryos responded better when precultured in B5m medium supplemented with 1 mg l−1 GA3, and fully mature embryos were capable of germinating directly in solid M1 medium. Our results suggest the existence of two types of dormancy in avocado embryos: an embryo-dormancy caused by cotyledons, and another type of dormancy, mainly occurring in 35 mm long embryos and revealed by the formation of dwarfing rosette seedlings, that can be released by a GA3 pretreatment.
PB Elsevier
YR 2007
FD 2007
LK https://hdl.handle.net/10630/36271
UL https://hdl.handle.net/10630/36271
LA eng
NO C. Sánchez-Romero, R. Perán-Quesada, B. Márquez-Martín, A. Barceló-Muñoz, F. Pliego-Alfaro, In vitro rescue of immature avocado (Persea americana Mill.) embryos, Scientia Horticulturae, Volume 111, Issue 4, 2007, Pages 365-370, ISSN 0304-4238, https://doi.org/10.1016/j.scienta.2006.11.009
NO This work was supported by the Comisión Interministerial de Ciencia y Tecnología, Spain(Grant no. AGL 2004-07028 C03-03/AGR).
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 21 ene 2026