RT Journal Article
T1 SOX10 Single Transcription Factor-Based Fast and Efficient Generation of Oligodendrocytes from Human Pluripotent Stem Cells.
A1 García-León, Juan Antonio
A1 Kumar, Manoj
A1 Boon, Ruben
A1 Chau, David
A1 One, Jennifer
A1 Wolfs, Esther
A1 Eggermont, Kristel
A1 Berckmans, Pieter
A1 Gunhanlar, Nilhan
A1 de Vrij, Femke
A1 Lendemeijer, Bas
A1 Pavie, Benjamin
A1 Corthout, Nikky
A1 Kushner, Steven A
A1 Dávila-Cansino, José Carlos
A1 Lambrichts, Ivo
A1 Hu, Wei-Shou
A1 Verfaillie, Catherine M
K1 Esclerosis múltiple
AB Scarce access to primary samples and lack of efficient protocols to generate oligodendrocytes (OLs) from human pluripotent stem cells (hPSCs) are hampering our understanding of OL biology and the development of novel therapies. Here, we demonstrate that overexpression of the transcription factor SOX10 is sufficient to generate surface antigen O4-positive (O4+) and myelin basic protein-positive OLs from hPSCs in only 22 days, including from patients with multiple sclerosis or amyotrophic lateral sclerosis. The SOX10-induced O4+ population resembles primary human OLs at the transcriptome level and can myelinate neurons in vivo. Using in vitro OL-neuron co-cultures, myelination of neurons by OLs can also be demonstrated, which can be adapted to a high-throughput screening format to test the response of pro-myelinating drugs. In conclusion, we provide an approach to generate OLs in a very rapid and efficient manner, which can be used for disease modeling, drug discovery efforts, and potentially for therapeutic OL transplantation.
PB Elsevier
YR 2018
FD 2018-02
LK https://hdl.handle.net/10630/34482
UL https://hdl.handle.net/10630/34482
LA eng
NO Juan Antonio García-León, Manoj Kumar, Ruben Boon, David Chau, Jennifer One, Esther Wolfs, Kristel Eggermont, Pieter Berckmans, Nilhan Gunhanlar, Femke de Vrij, Bas Lendemeijer, Benjamin Pavie, Nikky Corthout, Steven A. Kushner, José Carlos Dávila, Ivo Lambrichts, Wei-Shou Hu, Catherine M. Verfaillie, SOX10 Single Transcription Factor-Based Fast and Efficient Generation of Oligodendrocytes from Human Pluripotent Stem Cells, Stem Cell Reports, Volume 10, Issue 2, 2018, Pages 655-672, ISSN 2213-6711, https://doi.org/10.1016/j.stemcr.2017.12.014
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026