RT Journal Article
T1 Diversity and distribution of Halomonas in Rambla Salada, a hypersaline environment in the southeast of Spain.
A1 Oueriaghli, Nahid
A1 González-Doménech, Carmen María
A1 Martínez-Checa, Fernando
A1 Muyzer, Gerard
A1 Ventosa, Antonio
A1 Quesada, Emilia
A1 Béjar, Victoria
K1 Desnitrificación
K1 Bacterias desnitrificantes
K1 Salinidad
AB We have studied the diversity and distribution of Halomonas populations in the hypersaline habitat Rambla Salada (Murcia, southeastern Spain) by using different molecular techniques. Denaturing gradient gel electrophoresis (DGGE) using specific primers for the 16S rRNA gene of Halomonas followed by a multivariate analysis of the results indicated that richness and evenness of the Halomonas populations were mainly influenced by the season. We found no significant differences between the types of samples studied, from either watery sediments or soil samples. The highest value of diversity was reached in June 2006, the season with the highest salinity. Furthermore, canonical correspondence analysis (CCA) demonstrated that both salinity and pH significantly affected the structure of the Halomonas community. Halomonas almeriensis and two denitrifiers, H. ilicicola and H. ventosae were the predominant species. CARD-FISH showed that the percentage of Halomonas cells with respect to the total number of microorganisms ranged from 4.4% to 5.7%. To study the functional role of denitrifying species, we designed new primer sets targeting denitrification nirS and nosZ genes. Using these primers, we analyzed sediments from the upwelling zone collected in June 2006, where we found the highest percentage of denitrifiers (74%). Halomonas ventosae was the predominant denitrifier in this site.
PB Oxford University Press-Wiley
YR 2014
FD 2014-02-01
LK https://hdl.handle.net/10630/29131
UL https://hdl.handle.net/10630/29131
LA eng
NO Oueriaghli N, González-Domenech CM, Martínez-Checa F, Muyzer G, Ventosa A, Quesada E, Béjar V. Diversity and distribution of Halomonas in Rambla Salada, a hypersaline environment in the southeast of Spain. FEMS Microbiol Ecol. 2014 Feb;87(2):460-74. doi: 10.1111/1574-6941.12237
NO This research was supported by grants from the Ministry of Technology and Science (CGL2008-02399 and BIO2011-12879-E) and from the Plan Andaluz de Investigaci on (P06-CVI-01850), Spain.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 24 ene 2026