RT Journal Article
T1 Characterization of Curtovirus V2 Protein, a Functional Homolog of Begomovirus V2
T2 Characterization of Curtoviral V2 Protein
A1 Pérez-Luna, Ana Isabel
A1 Romero-Rodríguez, Beatriz
A1 Rosas-Díaz, Tábata Victoria
A1 Cerero, Laura
A1 Rodríguez Negrete, Edgar A.
A1 Castillo-Garriga, Araceli
A1 Rodríguez-Bejarano, Eduardo
K1 Patogénesis
AB Geminiviruses are single-stranded DNA plant viruses with circular genomes packagedwithin geminate particles. Among the Geminiviridae family, Begomovirus and Curtoviruscomprise the two best characterized genera. Curtovirus and Old World begomoviruspossess similar genome structures with six to seven open-reading frames (ORF). Amongthem, begomovirus and curtovirus V2 ORFs share the same location in the viral genome,encode proteins of similar size, but show extremely poor sequence homology betweenthe genera. V2 from Beet curly top virus (BCTV), the model species for the Curtovirusgenus, as it begomoviral counterpart, suppresses post-transcriptional gene silencing(PTGS) by impairing the RDR6/SGS3 pathway and localizes in the nucleus spanningfrom the perinuclear region to the cell periphery. By aminoacid sequence comparisonwe have identified that curtoviral and begomoviral V2 proteins shared two hydrophobicdomains and a putative phosphorylation motif. These three domains are essential forBCTV V2 silencing suppression activity, for the proper nuclear localization of the proteinand for systemic infection. The lack of suppression activity in the mutated versions ofV2 is complemented by the impaired function of RDR6 in Nicotiana benthamiana butthe ability of the viral mutants to produce a systemic infection is not recovered in genesilencing mutant backgrounds. We have also demonstrated that, as its begomoviralhomolog, V2 from BCTV is able to induce systemic symptoms and necrosis associatedwith a hypersensitive response-like (HR-like) when expressed from Potato virus X vectorin N. benthamiana, and that this pathogenicity activity does not dependent of its abilityto supress PTGS
PB Frontiers
YR 2020
FD 2020-06-19
LK https://hdl.handle.net/10630/34088
UL https://hdl.handle.net/10630/34088
LA eng
NO Luna AP, Romero-Rodríguez B, Rosas-Díaz T, Cerero L, Rodríguez-Negrete EA, Castillo AG and Bejarano ER (2020) Characterization of Curtovirus V2 Protein, a Functional Homolog of Begomovirus V2. Front. Plant Sci. 11:835. doi: 10.3389/fpls.2020.00835
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026