RT Journal Article
T1 Maslinic acid conjugate with 7-amino-4-methylcoumarin as probe to monitor the temperature dependent conformational changes of human serum albumin by FRET
A1 Molina-Bolívar, José Antonio
A1 Galisteo González, Francisco
A1 Carnero-Ruiz, Cristóbal
A1 Medina O´Donnell, Marta
A1 Martinez, Antonio
A1 Parra, Andrés
K1 Albuminuria
AB methylcoumarin is reported. Itwas found that the coumarin-maslinic derivative (MaCo) forms an excellent fluorescenceresonance energy transfer (FRET) pair with the tryptophan (Trp) residue of human serum albumin(HSA). This feature allowed for monitoring HSA conformational alterations by measuring the distance betweendonor (Trp) and acceptor (MaCo) through Förster energy transfer mechanism. Displacement experiments confirmedthat MaCo binds to subdomain IIA of HSA with independence of temperature. It was observed that, inthe temperature range 35–45 °C, the fluorescence emission maximumofHSA-MaCo complex decreased,whereasin the range 45 °C–65 °C, an incrementwas detected. The concomitant change in the polarity of environment surroundingTrp was confirmed by red edge excitation shift experiments. Thermal denaturation of HSA wasfollowed by time-resolved fluorescence spectroscopy. Average lifetime of Trp residue decreased with temperaturedue to the increment of solvent collisions and changes in the solvent exposure of Trp. To discriminate theimportance of each effect, lifetime of N-Acetyl-L-tryptophanamide (NATA) at different temperatures was measured.Circular dichroism(CD) studies confirmed the loss of secondary structure of HSA with increasing temperatureand showed a different trend in the conformational transformation below and above 45 °C, in agreementwith steady-state and time-resolved fluorescence experiments.
PB Elsevier
YR 2019
FD 2019
LK https://hdl.handle.net/10630/35603
UL https://hdl.handle.net/10630/35603
LA eng
NO J.A. Molina-Bolívar, F. Galisteo-González, C. Carnero Ruiz, M. Medina-O'Donnell, A. Martínez, A. Parra, Maslinic acid conjugate with 7-amino-4-methylcoumarin as probe to monitor the temperature dependent conformational changes of human serum albumin by FRET, Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, Volume 214, 2019, Pages 161-169, ISSN 1386-1425, https://doi.org/10.1016/j.saa.2019.02.014
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 24 ene 2026