RT Journal Article
T1 Identification of Reference Genes for Quantitative RT-PCR in Ascending Aortic Aneurysms.
A1 Henn, Dominic
A1 Bandner-Risch, Doris
A1 Perttunen, Hilja
A1 Schmied, Wolfram
A1 Porras-Martín, Carlos
A1 Rodríguez-Losada, Noela
A1 Schäfers, Hans-Joachim
K1 Aneurismas aórticos
AB Hypertension and congenital aortic valve malformations are frequent causes of ascending aortic aneurysms. The molecularmechanisms of aneurysm formation under these circumstances are not well understood. Reference genes for gene activitystudies in aortic tissue that are not influenced by aortic valve morphology and its hemodynamic consequences, aorticdilatation, hypertension, or antihypertensive medication are not available so far. This study determines genes in ascendingaortic tissue that are independent of these parameters. Tissue specimens from dilated and undilated ascending aortas wereobtained from 60 patients (age #70 years) with different morphologies of the aortic valve (tricuspid undilated n = 24,dilated n = 11; bicuspid undilated n = 6, dilated n = 15; unicuspid dilated n = 4). Of the studied individuals, 36 hadhypertension, and 31 received ACE inhibitors or AT1 receptor antagonists. The specimens were obtained intraoperativelyfrom the wall of the ascending aorta. We analyzed the expression levels of 32 candidate reference genes by quantitative RTPCR (RT-qPCR). Differential expression levels were assessed by parametric statistics. The expression analysis of these 32genes by RT-qPCR showed that EIF2B1, ELF1, and PPIA remained constant in their expression levels in the differentspecimen groups, thus being insensitive to aortic valve morphology, aortic dilatation, hypertension, and medication withACE inhibitors or AT1 receptor antagonists. Unlike many other commonly used reference genes, the genes EIF2B1, ELF1, andPPIA are neither confounded by aortic comorbidities nor by antihypertensive medication and therefore are most suitable forgene expression analysis of ascending aortic tissue.
PB PLOS
YR 2013
FD 2013
LK https://hdl.handle.net/10630/35670
UL https://hdl.handle.net/10630/35670
LA eng
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026