RT Journal Article
T1 Cost-Effective Markers and Candidate Genes Analysis at Wheat MQTL Loci.
A1 Mérida García, Rosa
A1 Gálvez-Rojas, Sergio
A1 Paux, Etienne
A1 Dorado-Pérez, Gabriel
A1 Pascual, Laura
A1 Giraldo, Patricia
A1 Hernández Molina, Pilar
K1 Trigo - Aspectos moleculares
AB High-resolution melting analysis (HRM) is a resolutive technique, using PCR amplification and in-tube detection, which is based on the PCR product’s melting analysis. It is a promising technique for breeding analysis, as it does not require dedicated sequencing equipment. It can be performed using QRT-PCR equipment that can be available in small-medium molecular biology laboratories or locally by the breeders, and it does not require an electrophoretic step to analyze the amplified DNA fragments. To develop effective HRM assays, the search for highly polymorphic sites amenable to PCR amplification is a prerequisite, which is not an easy task in wheat due to its genome complexity. The insertion site-based polymorphism markers (ISBPs) are PCR markers designed based on the knowledge of the sequence flanking transposable element (TE) sequences. The two PCR primers are designed with one in the transposable element and the other in the flanking DNA sequence. TEs are very abundant and nested in the wheat genome, with unique (genome-specific) insertion sites that are highly polymorphic. In this work, we analyze the available HRM-ISBP assays for wheat 3B and 4A chromosomes, and update their applications in wheat diversity at drought and heat MQTL loci.
PB MDPI
YR 2020
FD 2020-11-30
LK https://hdl.handle.net/10630/36176
UL https://hdl.handle.net/10630/36176
LA eng
NO This research was funded by project P18-RT-992 from Junta de Andalucía (Andalusian Regional Government), Spain (Co-funded by FEDER), and by the Spanish Ministry of Science and Innovation project PID2019-109089RB-C32. The marker Xgwm685 sequence was kindly provided by Marion Roeder, IPK Gatersleben.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026