RT Journal Article
T1 PipB2 is a substrate of the Salmonella pathogenicity island 1- encoded type III secretion system
A1 Baisón-Olmo, Fernando
A1 Cardenal Muñoz, Elena
A1 Ramos Morales, Francisco
K1 Salmonella
AB Salmonella harbors two type III secretion systems, T3SS1 and T3SS2, encoded on thepathogenicity islands SPI1 and SPI2, respectively. Several effector proteins are secretedthrough these systems into the eukaryotic host cells. PipB2 is a T3SS2 effector thatcontributes to the modulation of kinesin-1 motor complex activity. Here, we show thatPipB2 is also a substrate of T3SS1. This result was obtained infecting human epithelialHeLa cells for 2 hours and was confirmed in murine RAW264.7 macrophages, and ratNRK fibroblasts. Analysis at different time points after infection revealed thattranslocation of PipB2 is T3SS1-dependent in epithelial cells throughout the infection.In contrast, translocation into macrophages is T3SS1-dependent during invasion butT3SS2-dependent at later time points. The N-terminal 10 amino acid residues containthe signal necessary for translocation through both systems. These results confirm thefunctional overlap between these virulence-related secretion systems and suggest a newrole for the effector PipB2
PB Elvervier
YR 2012
FD 2012-06-29
LK https://hdl.handle.net/10630/37375
UL https://hdl.handle.net/10630/37375
LA eng
NO Baisón-Olmo F, Cardenal-Muñoz E, Ramos-Morales F. PipB2 is a substrate of the Salmonella pathogenicity island 1-encoded type III secretion system. Biochem Biophys Res Commun. 2012 Jun 29;423(2):240-6. doi: 10.1016/j.bbrc.2012.05.095. Epub 2012 May 26. PMID: 22640733.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 19 ene 2026