RT Journal Article
T1 Oxidative Stress and Inflammatory Biomarkers in Aqueous Humor and Blood of Patients with Leber's Hereditary Optic Neuropathy
A1 Sanchez-Fernández, Berta
A1 Zamorano-González, Pablo
A1 Martín-Montañez, Elisa
A1 Alba-Linero, Carmen
A1 Ríus-Díaz, Francisca
A1 García-Fernández, María Inmaculada
A1 Luque-Aranda, Rafael
A1 García-Basterra, Ignacio
K1 Nervio óptico - Enfermedades
AB Leber’s hereditary optic neuropathy (LHON) is a mitochondrial disorder that causes visual impairment due to the degeneration of retinal ganglion cells. Oxidative stress (OS) andinflammatory cytokines have been implicated in its pathophysiology. We investigated, for the first time, the presence of OS biomarkers and inflammatory cytokines in the aqueoushumor and peripheral blood of LHON patients compared to controls, aiming to identify potential clinical biomarkers for diagnosis and disease monitoring. A total of 38 participantswere enrolled in a single-center, retrospective observational study, including 17 genetically confirmed LHON patients from different Spanish regions and 21 controls. OS biomarkersand inflammatory cytokines were quantified using spectrophotometry and fluorimetry techniques. Statistical analyses were performed to compare groups and to assess thediscriminatory performance of biomarkers in identifying affected individuals. Compared to controls, LHON patients exhibited significantly higher levels of AOPP, LOOH, nitrotyrosine,GPX, GRD, and OX/AntiOX ratio in both aqueous humor and serum. Among these, serum LOOH levels and the OX/AntiOX ratio were the most reliable for identifying patientsaffected, with high sensitivity and specificity. However, additional data on serum IL-1ra are required to confirm its potential as an effective classifier. These findings highlight novelcandidate biomarkers for the diagnosis and monitoring of LHON progression
PB MDPI
YR 2025
FD 2025-12-30
LK https://hdl.handle.net/10630/45044
UL https://hdl.handle.net/10630/45044
LA eng
NO Sánchez-Fernández B, Zamorano-González P, Martín-Montañez E, Alba-Linero C, Rius-Díaz F, García-Fernandez M, Luque-Aranda R, García-Basterra I. Oxidative Stress and Inflammatory Biomarkers in Aqueous Humor and Blood of Patients with Leber's Hereditary Optic Neuropathy. Antioxidants (Basel). 2025 Dec 30;15(1):51.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 4 mar 2026