RT Journal Article
T1 Transcriptional profiling of dendritic cells in a mouse model of food-antigen induced anaphylaxis reveals the upregulation of multiple immune-related pathways.
A1 Rodríguez, María José
A1 Palomares, Francisca
A1 Bogas, Gador
A1 Torres-Jaén, María Josefa
A1 Díaz-Perales, Araceli
A1 Rojo, Javier
A1 Plaza Serón, María del Carmen
A1 Rodríguez-Nogales, Alba
A1 Orengo, Christine A.
A1 Mayorga Mayorga, Cristobalina
A1 Perkins, James Richard
K1 Anafilaxia
K1 Células dendríticas
K1 Alergia a los alimentos
K1 Proteínas transportadoras
AB ScopeMuch of our knowledge about gene expression during anaphylaxis comes from candidate gene studies. Despite their potential role, expression changes in dendritic cells (DCs) have not been studied in this context using high throughput methods. We investigated the molecular mechanisms underlying food-antigen induced anaphylaxis using DCs from an animal model.Methods and resultsWe used RNA sequencing to study gene expression in lymph node derived DCs from anaphylactic mice sensitized intranasally with the major peach allergen Pru p 3, during the acute reaction phase, induced intraperitoneally. In total, 237 genes changed significantly, 181 showing at least two-fold changes. Almost three quarters of these increased during anaphylaxis. A subset was confirmed using RT-PCR in a second set of samples obtained from a new batch of mice. Enrichment analysis showed an overrepresentation of genes involved in key immune system and inflammatory processes including TGF-β signalling. Comparison with a study using anaphylactic human subjects showed significant overlap.ConclusionsOur findings provide a comprehensive overview of the transcriptional changes occurring in DCs during anaphylaxis and help elucidate the mechanisms involved. They add further weight to the putative role of these cells in anaphylaxis and highlights genes that may represent potential therapeutic targets.
PB Wiley
YR 2018
FD 2018-10-20
LK https://hdl.handle.net/10630/34461
UL https://hdl.handle.net/10630/34461
LA eng
NO Rodriguez, M. J., Palomares, F., Bogas, G., Torres, M. J., Diaz‐Perales, A., Rojo, J., Plaza‐Seron, M. del C., Rodriguez‐Nogales, A., Orengo, C., Mayorga, C., & Perkins, J. R. (2019). Transcriptional Profiling of Dendritic Cells in a Mouse Model of Food‐Antigen‐Induced Anaphylaxis Reveals the Upregulation of Multiple Immune‐Related Pathways. Molecular Nutrition & Food Research, 63(3).
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 19 ene 2026