RT Conference Proceedings
T1 “Off-on” two-photon indolenine probes for pH bioimaging
A1 Benítez-Martín, Carlos
A1 Guadix-Domínguez, Juan Antonio
A1 Pearson, John R.
A1 Nájera-Albendín, Francisco
A1 Pérez-Pomares, José María
A1 Pérez-de-Inestrosa-Villatoro, Ezequiel
K1 Biodetectores
K1 Microscopía de fluorescencia
K1 Sondas fluorescentes
K1 Iones hidrógeno - Concentración
AB Fluorescence-based biosensors have become an indispensable tool in modern biology. These molecules can be used to monitor processes inside living cells and in real-time. The control of the pH is crucial in multiple biological process such as proliferation, apoptosis and defense. Therefore, the use of such pH probes are widespread in fluorescence microscopy.Recently, we have described a biosensor with excellent photophysical and suitable two-photon absorption (TPA) properties. This sensor allows the detection of hydroxyl radicals inside lysosomes. Based on this scaffold, we have designed, synthesized and characterized a new TPA fluorescent probe with an “off-on” response to different pH environments. These naphthalene-indolenine derivatives have a high synthetic versatility through affordable and efficient synthesis. The preliminary results suggest that this sensor has promising properties for detecting pH changes inside living cells.
YR 2019
FD 2019-10-25
LK https://hdl.handle.net/10630/18641
UL https://hdl.handle.net/10630/18641
LA eng
NO Real Sociedad Española de Química. Universidad de Murcia. Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 4 mar 2026