RT Journal Article
T1 Selection of putative probiotics based on antigen-antibody cross-reaction with Photobacterium damselae subsp. piscicida and Vibrio harveyi for use in Senegalese sole (Solea senegalensis)
A1 Medina, Alberto
A1 Moriñigo-Gutiérrez, Miguel Ángel
A1 Arijo-Andrade, Salvador
K1 Probióticos
AB Probiotics can be used to reduce disease outbreaks in aquaculture. Some of them are characterised by theirantagonistic activity against pathogens or the stimulation of the fish immune response, including the productionof specific antibodies. If a probiotic has common antigens with a determined pathogen, it could produce antibodieswith a cross-reaction to that pathogen. Thus, a probiotic with these characteristics could be used in asimilar way to a live vaccine. The aim of this study was to select bacteria with antigenic similarity and antagonisticactivity against the pathogens Photobacterium damselae subsp. piscicida and Vibrio harveyi, and to determinetheir ability to stimulate the production of antibodies in sole (Solea senegalensis, Kaup) with crossreactionagainst these pathogens.Dot blot was used to detect strains with cross-reaction using sera immunized against P. damselae subsp.piscicida Lg41.01 and V. harveyi Lg16.00. A total of 138 strains were selected from 718 strains, based on theintensity of the dot blot reaction. A second selection was performed to detect their ability to inhibit the pathogensgrowth. Five strains inhibited the growth of P. damselae subsp. piscicida, four strains inhibited the growthof V. harveyi, while two strains inhibited both pathogens. A Western blot confirmed the cross-reactions of theselected strains with the pathogens.Selected strains were subsequently inoculated into sole specimens by intraperitoneal injection. Four strainsproduced antibodies with cross-reaction against the pathogens. None mortality was observed in the inoculatedfish. Further research demonstrated the storage capability of the selected strains in saline solution and feed, theirgrowth at low pH, and identified their enzymatic characteristics. In conclusion, the selected strains showedantimicrobial activity and capacity to activate a specific immune response against fish pathogens.
PB Elsevier
YR 2020
FD 2020-07
LK https://hdl.handle.net/10630/29887
UL https://hdl.handle.net/10630/29887
LA eng
NO Alberto Medina, Miguel Ángel Moriñigo, Salvador Arijo, Selection of putative probiotics based on antigen-antibody cross-reaction with Photobacterium damselae subsp. piscicida and Vibrio harveyi for use in Senegalese sole (Solea senegalensis), Aquaculture Reports, Volume 17, 2020, 100366, ISSN 2352-5134, https://doi.org/10.1016/j.aqrep.2020.100366. (https://www.sciencedirect.com/science/article/pii/S2352513420301629)
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026