RT Journal Article
T1 Diversity Arrays Technology (DArT) marker platforms for diversity analysis and linkage mapping in a complex crop, the octoploid cultivated strawberry (Fragaria × ananassa)
A1 Sánchez-Sevilla, José Federico
A1 Hovath, Aniko
A1 Botella-Mesa, Miguel Ángel
A1 Gaston, Amèlia
A1 Folta, Kevin
A1 Kilian, Andrzej
A1 Denoyes, Beatrice
A1 Amaya, Iraida
K1 Fresas - Aspectos moleculares
AB Cultivated strawberry (Fragaria × ananassa) is a genetically complex allo-octoploid crop with28 pairs of chromosomes (2n = 8x = 56) for which a genome sequence is not yet available.The diploid Fragaria vesca is considered the donor species of one of the octoploid subgenomesand its available genome sequence can be used as a reference for genomic studies.A wide number of strawberry cultivars are stored in ex situ germplasm collections worldwidebut a number of previous studies have addressed the genetic diversity present within alimited number of these collections. Here, we report the development and application of twoplatforms based on the implementation of Diversity Array Technology (DArT) markers forhigh-throughput genotyping in strawberry. The first DArT microarray was used to evaluatethe genetic diversity of 62 strawberry cultivars that represent a wide range of variationbased on phenotype, geographical and temporal origin and pedigrees. A total of 603 DArTmarkers were used to evaluate the diversity and structure of the population and their clusteranalyses revealed that these markers were highly efficient in classifying the accessions ingroups based on historical, geographical and pedigree-based cues. The second DArTseqplatform took benefit of the complexity reduction method optimized for strawberry and thedevelopment of next generation sequencing technologies. The strawberry DArTseq wasused to generate a total of 9,386 SNP markers in the previously developed ‘232’ × ‘1392’mapping population, of which, 4,242 high quality markers were further selected to saturatethis map after several filtering steps. The high-throughput platforms here developed for genotypingstrawberry will facilitate genome-wide characterizations of large accessions setsand complement other available options.
PB PLOS One
YR 2015
FD 2015-12-16
LK https://hdl.handle.net/10630/37268
UL https://hdl.handle.net/10630/37268
LA eng
NO Sánchez-Sevilla JF, Horvath A, Botella MA, Gaston A, Folta K, Kilian A, et al. (2015) Diversity Arrays Technology (DArT) Marker Platforms for Diversity Analysis and Linkage Mapping in a Complex Crop, the Octoploid Cultivated Strawberry (Fragaria × ananassa). PLoS ONE 10(12): e0144960. https://doi.org/10.1371/journal.pone.0144960
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 24 ene 2026