RT Journal Article
T1 Evidence for horizontal gene transfer, gene duplication and genetic variation as driving forces of the diversity of haemolytic phenotypes in Photobacterium damselae subsp. damselae
A1 Rivas, Amable
A1 Labella Vera, Alejandro Manuel
A1 Borrego, Juan José
A1 Lemos, Manuel
A1 Osorio, Carlos
K1 Bacterias marinas
AB Photobacterium damselae subsp. damselae, a marine bacterium that causesinfections in marine animals and in humans, produces up to three differenthaemolysins involved in virulence, which include the pPHDD1 plasmidencodeddamselysin (Dly) and HlyApl, and the chromosome-encoded HlyAch.We screened 45 isolates from different origins, and found a correlation betweentheir haemolytic phenotypes and the differential haemolysin gene content. Allhighly and medium haemolytic strains harboured pPHDD1, with amino acidsubstitutions in HlyApl and HlyAch being the cause of the medium haemolyticphenotypes in some pPHDD1-harbouring strains. Weakly haemolytic strainscontained only hlyAch, whereas nonhaemolytic isolates, in addition to lackingpPHDD1, either lacked hlyAch or contained a hlyAch pseudogene. Sequenceanalysis of the genomic context of hlyAch uncovered an unexpected geneticdiversity, suggesting that hlyAch is located in an unstable chromosomal region.Phylogenetic analysis suggested that hlyApl and hlyAch originated by gene duplicationwithin P. damselae subsp. damselae following acquisition by horizontaltransfer. These observations together with the differential distribution ofpPHDD1 plasmid among strains suggest that horizontal gene transfer hasplayed a main role in shaping the haemolysin gene baggage in this pathogen.
PB Oxford Academic
YR 2014
FD 2014-06-01
LK https://hdl.handle.net/10630/33056
UL https://hdl.handle.net/10630/33056
LA eng
NO Amable J. Rivas, Alejandro M. Labella, Juan J. Borrego, Manuel L. Lemos, Carlos R. Osorio, Evidence for horizontal gene transfer, gene duplication and genetic variation as driving forces of the diversity of haemolytic phenotypes in Photobacterium damselae subsp. damselae, FEMS Microbiology Letters, Volume 355, Issue 2, June 2014, Pages 152–162, https://doi.org/10.1111/1574-6968.12464
NO This work was supported by grant EM2012/043 from the Xunta de Galicia, Spain, and by grant AGL2012-39274- C02-01 from the Ministry of Economy and Competitive- ness (MINECO) of Spain (cofunded by the FEDER Programme from the European Union).
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 21 ene 2026