RT Journal Article
T1 Copper-containing mesoporous bioactive glass promotes angiogenesis in an in vivo zebrafish model
A1 Romero-Sánchez, Lilian B.
A1 Mari-Beffa, Manuel
A1 Carrillo Fernández, Paloma
A1 Medina-Torres, Miguel Ángel
A1 Díaz-Cuenca, Aránzazu
K1 Neovascularización
AB The osteogenic and angiogenic responses of organisms to the ionic products of degradation of bioactive glasses (BGs) are being intensively investigated. The promotion of angiogenesis by copper (Cu) has been known for more than three decades. This element can be incorporated to delivery carriers, such as BGs, and the materials used in biological assays. In this work, Cu-containing mesoporous bioactive glass (MBG) in the SiO2-CaO-P2O5 compositional system was prepared incorporating 5% mol Cu (MBG-5Cu) by replacement of the corresponding amount of Ca. The biological effects of the ionic products of MBGbiodegradation were evaluated on a well-known endothelial cell line, the bovine aorta endothelial cells (BAEC), as well as in an in vivo zebrafish (Danio rerio) embryo assay. The results suggest that ionic products of both MBG (Cu free) and MBG-5Cu materials promote angiogenesis. In vitro cell cultures show that the ionic dissolution products of these materials are not toxic and promote BAEC viability and migration.In addition, the in vivo assay indicates that both exposition and microinjection of zebrafish embryos with Cu free MBG material increase vessel number and thickness of the subintestinal venous plexus (SIVP), whereas assays using MBG-5Cu enhance this effect.
YR 2018
FD 2018-10-19
LK https://hdl.handle.net/10630/16663
UL https://hdl.handle.net/10630/16663
LA eng
NO The authors gratefully acknowledge the financial support provided by the Andalusian Ministry of Economy, Science and Innovation(Proyectos Excelencia Grants no. P10-CTS-6681 and no. P12-CTS-1507) and Spanish Ministry of Economy and Competitivity(BIO2014-56092-R). LBRS acknowledges the CONACYT-Mexico Fellowship PhD Program.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026