RT Conference Proceedings
T1 Geminiviral protein Rep interferes in PCNA sumoylation
A1 Sabarit Penalosa, Blanca
A1 Arroyo-Mateos, Manuel
A1 Sánchez-Durán, Miguel A.
A1 Rodríguez-Bejarano, Eduardo
K1 Virus fitopatógenos
AB Rep is a multifunctional protein essential for replication of geminivirus that interferes with the sumoylation of a key protein in the DNA replication, PCNA (Proliferating Cell Nuclear Antigen). It is known that Rep is capable of interacting with a plethora of plant proteins, including PCNA. Despite the biological significance remains unknown, it’s thought that this interaction should play a key role for generating new copies of the virus genome. Therefore, in order to characterize this interaction, we study which lysines are sumoylated in tomato PCNA (SlPCNA). Considering conservation, location and presence of sumoylation domain criteria, we have identified some candidate lysines and studied how its mutation affects this protein sumoylation in Escherichia coli assays. Finally, we plan to confirm and characterize the Rep interference on SlPCNA sumoylation and determine if this interference occurs in planta.
YR 2015
FD 2015-10-21
LK http://hdl.handle.net/10630/10558
UL http://hdl.handle.net/10630/10558
LA eng
NO Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 21 ene 2026