RT Journal Article
T1 Psychrobacillus vulpis sp. nov., a new species isolated from faeces of a red fox in Spain.
A1 Rodríguez-González, Miguel Ángel
A1 Reina, José Carlos
A1 Béjar, Victoria
A1 Llamas, Inmaculada
K1 Baciláceas
K1 Genomas
K1 Quimiotaxonomía
K1 Filogenia
K1 Zorro rojo - Enfermedades bacterianas
AB A facultative anaerobic, chemoheterotrophic, endospore-forming, Gram-stain-positive rod, designated as strain Z8T, was isolated from red fox (Vulpes vulpes) faeces sampled at Tablas de Daimiel National Park, Ciudad Real, Spain. Strain Z8T grewat 0–37°C (optimum, 28°C), in the presence of 0–5.5% (w/v) NaCl (2.5%, w/v) and at pH 6–10 (pH 7). The strain was motileand positive for catalase, oxidase, H2S and siderophore production, acid and alkaline phosphatases, and N-acetylglucosamine,adipic acid and malate assimilation. It hydrolysed starch, DNA, l-tyrosine, Tween 20, Tween 80 and lecithovitellin. Phylogeneticanalysis of the 16S rRNA gene sequence indicated that strain Z8T is a member of the genus Psychrobacillus, showing highsequence similarity to Psychrobacillus lasiicapitis NEAU-3TGS17T (99.2%) and Psychrobacillus soli NHI-2TT (99.1%), and around98% to other known species of the genus Psychrobacillus. Digital DNA–DNA hybridization and average nucleotide identity valueswere lower than 24 and 79%, respectively, with the most related species. In silico G+C content was 35.9mol%. The major cellularfatty acids of strain Z8T were iso-C14:0, iso-C15:0 and anteiso-C15:0. The novel strain contained diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol as predominant polar lipids, and the main respiratory isoprenoid quinone was MK-8.Based on the 16S rRNA phylogenetic analysis, together with MLSA (recA, rpoB and gyrB), phylogenomic, chemotaxonomic andphenotypic results, we demonstrate that strain Z8T represents a novel species of the genus Psychrobacillus, for which the namePsychrobacillus vulpis sp. nov., is proposed. The type strain is Z8T (=CECT 9721T=LMG 31001T).
PB Miicrobiology Society
YR 2019
FD 2019-12-13
LK https://hdl.handle.net/10630/40462
UL https://hdl.handle.net/10630/40462
LA eng
NO Rodríguez et al., Int. J. Syst. Evol. Microbiol. 2020;70:882–888 DOI 10.1099/ijsem.0.003840
NO https://openpolicyfinder.jisc.ac.uk/id/publication/14638
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026