RT Conference Proceedings
T1 Galanin n-terminal gragment (1-15) modifies the 5-HT1A receptor against [H3]-8-OH-DPAT binding in the dorsal raphe and hippocampus of the rat
A1 Flores-Burgess, Antonio
A1 Millón-Peñuela, Carmelo
A1 Narváez-Peláez, Manuel
A1 Barroto-Escuela, Dasiel
A1 Santín-Núñez, Luis Javier
A1 Gago-Calderón, Belén
A1 Gomez de Travecedo, Isabel
A1 Narváez-Bueno, José Ángel
A1 Fuxe, Kjell
A1 Díaz-Cabiale, Zaida
K1 Hipocampo (Cerebro)
K1 Neuropéptidos
AB We have described that Galanin N-terminal fragment (1-15) [GAL(1-15)] is associated with depressive effects and also modulates the antidepressant effects induced by the 5-HT1A receptor (5-HT1AR) agonist 8-OH-DPAT. The aim of this study is to analyze the ability of GAL(1-15) to modulate 5-HT1AR at the autoreceptor and postsynaptic receptor level in rats by using quantitative autoradiography.We analyzed the effect of intracerebroventricular GAL(1-15)-3nmol (n=6) or aCSF (n=6), 10 minutes, 2 and 5 hours after the injection, on the binding characteristics of the 5-HT1AR agonist [H3]-8-OH-DPAT in sections of the Dorsal Raphe (DR) and Dorsal Hippocampus, specifically CA1 and Dentate Gyrus (DG). Student’s t-test was used to compare the experimental groups.GAL(1-15) produced a time-dependent effect on the binding of [H3]-8-OH-DPAT. In CA1 and DG, a significant increase in the KD and Bmax was observed, by 90%(p<0.05), at 10 minutes and 2 hours after injection. However, 5 hours after GAL(1-15) the only significant change remaining was the increase in Bmax at the DG.The coinjection of the GALR2 antagonist M871 blocked significantly the effects induced by GAL(1-15) in both areas. In DR, 2 hours after injection GAL(1-15) only produced a decrease in the Bmax by  20%(p<0.05).These results indicate that GAL(1-15) interacts with 5-HT1AR at the receptor level in DR and Dorsal Hippocampus. Therapeutic strategies based on these results could be developed for the treatment of depression disorders.This work has been supported by Junta de Andalucia CVI646 and SpanishMinistry of Economy PSI2013-44901-P.
YR 2016
FD 2016-09-09
LK http://hdl.handle.net/10630/11970
UL http://hdl.handle.net/10630/11970
LA eng
NO Poster en Congreso
NO Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 7 mar 2026