RT Journal Article
T1 Investigation of the diagnostic accuracy of basophil activation test for allergic phenotypes of rhinitis
A1 Testera Montes, Almudena
A1 Ariza Veguillas, Adriana
A1 Sola-Martínez, Rosa Alba
A1 Aranda, Carlos José
A1 Bentabol-Ramos, Guillermo
A1 Sánchez-Torralvo, Dulce
A1 Mayorga Mayorga, Cristobalina
A1 Torres-Jaén, María José
A1 Eguiluz-Gracia, Ibón
A1 Rondón Segovia, Carmen
K1 Basófilos
K1 Alergia
AB Introduction: Basophil activation test (BAT) might be an alternative to nasal allergenchallenge (NAC) to identify the allergic etiology in rhinitis patients. Here, we investi-gate the diagnostic performance of BAT for allergic phenotypes of rhinitis.Methods: Rhinitis patients and healthy controls were subjected to NAC withDermatophagoides pteronyssinus (DP), Alternaria alternata (AA), grass (GP) and olive(OP) pollens. Rhinitis subjects also underwent skin prick test (SPT) with relevant al-lergens. Patients were classified into allergic rhinitis (AR, positive NAC and SPT), localallergic rhinitis (LAR, positive NAC and negative SPT), dual allergic rhinitis (DAR, de-fined as AR for ≥1 allergen and LAR for ≥1 allergen), and non-allergic rhinitis (NAR,negative NAC and SPT) phenotypes. BAT with DP, AA, GP and OP was conducted instudy individuals and compared with NAC results.Results: A total of 47 AR, 31 DAR, 26 LAR, 12 NAR and 21 control subjects were re-cruited. The best positivity cut-offs of BAT for DP-, AA-, GP- and OP-driven allergy (allphenotypes) were a %CD63 cells of 8.650, 14.250, 26.200, and 12.780, respectively(AUC 0.851, 0.701, 0.887, and 0.921, respectively).Sensitivity, specificity, negative and positive predictive values of BAT (all pheno-types) ranged 43.5%(AA)-83.3%(OP), 88.9%(GP)-100%(AA), 87%(GP)-100%(AA), and61.1%(DP)-80.0%(pollens), respectively. BAT identified 79%–100% of SPT-positive aller-gies (AR and DAR), and 25%–75% of SPT-negative allergies (LAR and DAR), while ≤10%of NAR/HC subjects tested positive. BAT positivity correlated with rhinitis severity inLAR patients (p = 0.018), and associated with conjunctivitis (p = 0.015) in allergic subjects.
PB Wiley
YR 2024
FD 2024
LK https://hdl.handle.net/10630/35024
UL https://hdl.handle.net/10630/35024
LA eng
NO Testera-Montes A, Ariza A, Sola-Martinez RA, et al. Investigation of the diagnostic accuracy of basophil activation test for allergic phenotypes of rhinitis. Allergy. 2024; 00: 1-12. doi:10.1111/all.16390
NO Funding for open access charge: Universidad de Málaga / CBUA This study was supported by Instituto de Salud Carlos III (ISCIII) of the Spanish Ministry of Science and Competitiveness (grants co-funded by the European Regional Development Fund) through the research projects PI20/01715, PI23/01516 and PI23/00875. A.T-M. holds a “Juan Rodes” contract (JR22/00048), and G.B-R. holds a “Rio Hortega” contract (CM23/00092), whereas CJA was supported by a “Sara Borrell” contract (CD21/00034), all granted by ISCIII. R.A.S-M. holds a “Margarita Salas” contract (133/MSJD/22) granted by Universidad de Murcia in conjunction with the Spanish Ministry of Universities (grants co-funded by Next Generation EU and EU Recovery, Transformation, and Resilience Plan). IEG holds an ‘Accion B’ contract (B-0007-2023), and A.A. and CM hold ‘Nicolas Monardes’ contracts (C1-0007-2023 and RC-0004-2021, respectively) all granted by the Regional Ministry of Health of Andalucia (co-funded by European Social Fund (ESF) ‘Andalucia se mueve con Europa’). CJA holds a Marie Sklodowska-Curie Actions (MSCA) Postdoctoral Fellowship from Horizon Europe (101105416). This study was also supported by ISCIII (grants co-funded by the European Regional Development Fund) through its programme of Redes de Investigacion Cooperativa Orientadas al Resultado en Salud (RICORS): Enfermedades Inflamatorias (RD21/0002/0008).
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026