RT Conference Proceedings
T1 Unraveling the mechanism of TTL genes in cellulose biosynthesis
A1 García-Moreno, Álvaro
A1 Amorim-Silva, Vitor
A1 Castillo-Garriga, Araceli
A1 Menna, Alexandra
A1 Kesten, Christopher
A1 Valpuesta-Fernández, Victoriano
A1 Macho, Alberto P.
A1 Jaillais, Yvon
A1 Sánchez-Rodríguez, Clara
A1 Botella-Mesa, Miguel Ángel
K1 Biología
AB As sessile organisms, plants require mechanisms to sense and respond to the challenging environment, that encompass both biotic and abiotic factors that results in differential development. In these conditions is essential to balance growth and stress responses. As cell walls shape plant growth, this differential growth response cause alterations to the plant cell wall where cellulose is the major component. Therefore, understanding the mechanisms that regulate cellulose biosynthesis is essential to develop strategies to improve plant production. In Arabidopsis, the TETRATRICOPEPTIDE THIOREDOXIN-LIKE (TTL) gene family is composed by four members (TTL1 to TTL4) and mutations in TTL1, TTL3, and TTL4 genes cause reduced growth under salt and osmotic stress due to defects in plant cell wall integrity. We observe association of TTL3 with most core components in traducing BR signalling, such as LRR-RLK BRI1 or GSK3 BIN2 that modulate cellulose biosynthesis through phosphorylating cellulose synthases. Here, we show that ttl mutants present defects in the plant cell wall, particularly in Isoxaben, salt or sucrose stress. Spinning disk microscopy in etiolated hypocotyls reveals that, TTL proteins are responsible for the cellulose synthase complex (CSC) stability in plasma membrane (PM) upon sucrose stress. Moreover, TTL3 associates with LRR-RLKs that have been shown to be important for cellulose biosynthesis such as FEI1 in the FEI1/FEI2/SOS5 pathway. We aim to investigate the mechanisms by which TTL proteins regulate CesA stability in PM under stress, using a combination of genetics, biochemical, and molecular and cell biology approaches.
YR 2019
FD 2019-05-10
LK https://hdl.handle.net/10630/17636
UL https://hdl.handle.net/10630/17636
LA spa
NO Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.This work was supported by grants from: (1) Ministerio de Ciencia e Innovación BIO2014-55380-R, BIO2014-56153-REDT; (2) Ministerio de Economía, Industria y Competitividad (BES-2015-071256)
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026