RT Journal Article
T1 Live cell imaging reveals 3′-UTR dependent mRNA sorting to synapses
A1 Bauer, Karl E.
A1 Segura, Inmaculada
A1 Gaspar, Imre
A1 Scheuss, Volker
A1 Illig, Christin
A1 Ammer, Georg
A1 Hutten, Saskia
A1 Basyuk, Eugénia
A1 Fernández-Moya, Sandra M.
A1 Ehses, Janina
A1 Bertrand, Edouard
A1 Kiebler, Michael A.
K1 Ácido ribonucleico mensajero
AB mRNA transport restricts translation to specific subcellular locations, which is the basis for many cellular functions. However, the precise process of mRNA sorting to synapses inneurons remains elusive. Here we use Rgs4 mRNA to investigate 3′-UTR-dependent trans- port by MS2 live-cell imaging. The majority of observed RNA granules display 3′-UTRindependent bidirectional transport in dendrites. Importantly, the Rgs4 3′-UTR causes an anterograde transport bias, which requires the Staufen2 protein. Moreover, the 3′-UTRmediates dynamic, sustained mRNA recruitment to synapses. Visualization at high temporal resolution enables us to show mRNA patrolling dendrites, allowing transient interaction with multiple synapses, in agreement with the sushi-belt model. Modulation of neuronal activity by either chemical silencing or local glutamate uncaging regulates both the 3′-UTR-depen- dent transport bias and synaptic recruitment. This dynamic and reversible mRNA recruitment to active synapses would allow translation and synaptic remodeling in a spatially and tem- porally adaptive manner.
PB Springer Nature
YR 2019
FD 2019-07-18
LK https://hdl.handle.net/10630/45564
UL https://hdl.handle.net/10630/45564
LA eng
NO Bauer, K.E., Segura, I., Gaspar, I. et al. Live cell imaging reveals 3′-UTR dependent mRNA sorting to synapses. Nat Commun 10, 3178 (2019). https://doi.org/10.1038/s41467-019-11123-x
NO Deutsche Forschungsgemeinschaft
NO FWF
NO Friedrich-Baur-Stiftung
NO Agence nationale de la recherche
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 3 mar 2026