RT Journal Article
T1 Hexyltrimethylammonium ion enhances potential copper-chelating  properties of ammonium thiomolybdate in an in vivo zebrafish model.
A1 Vega-Granados, Karla
A1 Escobar-Ibarra, Paola
A1 Palomino-Vizcaino, Kenia
A1 Cruz-Reyes, Juan
A1 Valverde-Guillén, Piedad
A1 Latorre-Redoli, Sofía
A1 Caneda-Santiago, Candela T.
A1 Mari-Beffa, Manuel
A1 Romero-Sánchez, Lilian B.
K1 Peces cebra
K1 Voltametría
K1 Análisis electroquímico
K1 Quelatoterapia
K1 Cobre
AB Ammonium and hexyltrimethylammonium thiomolybdates (ATM and ATM-C6) and thiotungstates (ATT and ATT-C6) were synthesized. Their toxicity was evaluated using both in vitro and in vivo approaches via the zebrafish embryo acute toxicity assay (ZFET), while the copper-thiometallate interaction was studied using cyclic voltammetry, as well as in an in vivo assay. Cyclic voltammetry suggests that all thiometallates form complexes with copper in a 2:1 Cu:thiometallate ratio. Both in vitro and in vivo assays demonstrated low toxicity in BALB/ 3T3 cells and in zebrafish embryos, with high IC50 and LC 50 values. Furthermore, the hexyltrimethylammonium ion played a crucial role in enhancing viability and reducing toxicity during prolonged treatments for ATM and ATT. In particular, the ZEFT assay uncovered the accumulation of ATM in zebrafish yolk, averted by the incorporation of the hexyltrimethylammonium ion. Notably, the copper-thiometallate interaction assay highlighted the improved viability of embryos when cultured in CuCl2 and ATM-C6, even at high CuCl2 concentrations. The hatching assay further confirmed that copper-ATM-C6 interaction mitigates inhibitory effects induced by thiomolybdates and CuCl2 when administered individually. These results suggest that the incorporation of the hexyltrimethylammonium ion in ATM increase its ability to interact with copper and its potential application as a copper chelator.
PB Elsevier
YR 2024
FD 2024-06-25
LK https://hdl.handle.net/10630/40162
UL https://hdl.handle.net/10630/40162
LA eng
NO Vega-Granados, K., Escobar-Ibarra, P., Palomino-Vizcaino, K., Cruz-Reyes, J., Valverde-Guillén, P., Latorre-Redoli, S., Caneda-Santiago, C. T., Marí-Beffa, M., & Romero-Sánchez, L. B. (2024). Hexyltrimethylammonium ion enhances potential copper-chelating properties of ammonium thiomolybdate in an in vivo zebrafish model. Archives of biochemistry and biophysics, 758, 110077. https://doi.org/10.1016/j.abb.2024.110077
NO https://openpolicyfinder.jisc.ac.uk/id/publication/11269
NO Junta de Andalucía
NO NextGenerationEU
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 3 mar 2026