RT Journal Article
T1 Microalgae and cyanobacteria as microbial substrate and their influence on the potential postbiotic capability of a bacterial probiotic
A1 Domínguez Maqueda, Marta
A1 Pérez Gómez, Olivia
A1 García Márquez, Jorge
A1 Espinosa Ruíz, Cristóbal
A1 Cuesta, Alberto
A1 Esteban, Mª. Ángeles
A1 Alarcón López, Francisco Javier
A1 Cárdenas, Casimiro
A1 Tapia-Paniagua, Silvana Teresa
A1 Balebona-Accino, María del Carmen
A1 Moriñigo-Gutiérrez, Miguel Ángel
K1 Microbiología
K1 Bacterias
K1 Microorganismos
AB Postbiotics are metabolic by-products from microorganisms that providehealth benefits to the host. Their secretion can be influenced by variousconditions affecting bacterial metabolism. This study presents a novel ap-proach for producing potential postbiotics, specifically extracellular products(ECPs), from the probiotic strain Shewanella putrefaciens SpPdp11, grownunder different culture conditions. These conditions include aquafeed media,with partial or total microalgae/cyanobacteria replacement as the microbialsubstrate, as well as variations in temperature and growth phase. The use ofmicroalgae/cyanobacteria as substrates may represent a valuable strategyfor generating novel postbiotics with unique properties. The ECPs assessedwere evaluated for their in vitro cytotoxic, hydrolytic and antimicrobial ac-tivities. Three conditions (ECPs derived from aquafeed media with partial(FM2324 and FM1548) or total (M2324) microalgae/cyanobacteria replace-ment) were non- cytotoxic to various fish cell lines and hydrolysed key nutri-tional compounds (casein, lipids, amylase and gelatin). Proteomic analysisof these ECP conditions revealed common structural and regulatory DNA-associated proteins, while differentially expressed proteins were associatedwith amino acid metabolism and antioxidant system (FM2324 and FM1548)and chemotaxis system (M2324). The results highlight the potential of theselected postbiotics as feed additives for future in vivo studies, aligning withsustainable development for aquaculture.
PB Wiley
YR 2024
FD 2024
LK https://hdl.handle.net/10630/35314
UL https://hdl.handle.net/10630/35314
LA spa
NO Domínguez-Maqueda M, Pérez-Gómez O, García-Márquez J, Espinosa-Ruíz C, Cuesta A, Esteban MÁ, Alarcón-López FJ, Cárdenas C, Tapia-Paniagua ST, Balebona MC, Moriñigo MÁ. Microalgae and cyanobacteria as microbial substrate and their influence on the potential postbiotic capability of a bacterial probiotic. Microb Biotechnol. 2024 Nov;17(11):e70046. doi: 10.1111/1751-7915.70046. PMID: 39573896; PMCID: PMC11582085.
NO Funding for open access charge: Universidad de Málaga/CBUA
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026