RT Journal Article
T1 The iturin and fengycin families of lipopeptides are key factors in antagonism of Bacillus subtilis toward Podosphaera fusca.
A1 Romero-Hinojosa, Diego Francisco
A1 De-Vicente-Moreno, Antonio
A1 Rakotoaly, Rivo H.
A1 Dufour, Samuel E.
A1 Veening, Jan-Willem
A1 Arrebola-Díez, Eva María
A1 Cazorla-López, Francisco Manuel
A1 Kuipers, Oscar
A1 Paquot, Michel
A1 Pérez-García, Alejandro
K1 Cucurbitáceas -  Enfermedades y plagas
K1 Agentes de control biológico de plagas
K1 Hongos fitopatógenos - Control
AB Podosphaera fusca is the main causal agent of cucurbit powdery mildew in Spain. Four Bacillus subtilis strains, UMAF6614, UMAF6619, UMAF6639, and UMAF8561, with proven ability to suppress the disease on melon in detached leaf and seedling assays, were subjected to further analyses to elucidate the mode of action involved in their biocontrol performance. Cell-free supernatants showed antifungal activities very close to those previously reported for vegetative cells. Identification of three lipopeptide antibiotics, surfactin, fengycin, and iturin A or bacillomycin, in butanolic extracts from cell-free culture filtrates of these B. subtilis strains pointed out that antibiosis could be a major factor involved in their biocontrol ability. The strong inhibitory effect of purified lipopeptide fractions corresponding to bacillomycin, fengycin, and iturin A on P. fusca conidia germination, as well as the in situ detection of these lipopeptides in bacterial-treated melon leaves, provided interesting evidence of their putative involvement in the antagonistic activity. Those results were definitively supported by site-directed mutagenesis analysis, targeted to suppress the biosynthesis of the different lipopeptides. Taken together, our data have allowed us to conclude that the iturin and fengycin families of lipopeptides have a major role in the antagonism of B. subtilis toward P. fusca.
PB The American Phytopathological Society
YR 2007
FD 2007-03-15
LK https://hdl.handle.net/10630/31187
UL https://hdl.handle.net/10630/31187
LA eng
NO Romero D, de Vicente A, Rakotoaly RH, Dufour SE, Veening JW, Arrebola E, Cazorla FM, Kuipers OP, Paquot M, Pérez-García A. 2007. The iturin and fengycin families of lipopeptides are key factors in antagonism of Bacillus subtilis toward Podosphaera fusca. Mol Plant Microbe Interact. 20(4):430-40.
NO This study was supported by grants from Plan Nacional de Recursos y Tecnologías Agroalimentarias from the Ministerio de Educación y Ciencia, Spain (AGL2001-1837-C02-01 and AGL2004-06056-C02-01).
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 19 ene 2026