RT Journal Article
T1 Antiviral, Immunomodulatory and Antiproliferative Activities of Recombinant Soluble IFNAR2 without IFN-ß Mediation
A1 Hurtado-Guerrero, Isaac
A1 Hernáez, Bruno
A1 Pinto-Medel, Maria Jesús
A1 Calonge, Esther
A1 Rodríguez-Bada, José Luis
A1 Urbaneja, Patricia
A1 Alonso, Ana
A1 Mena-Vázquez, Natalia
A1 Aliaga, Pablo
A1 Issazadeh-Navikas, Shohreh
A1 Pavía-Molina, José
A1 Leyva-Fernández, Laura
A1 Alcamí, José
A1 Alcamí, Antonio
A1 Fernández-Fernández, Óscar
A1 Oliver-Martos, Begoña
K1 Inmunología
AB Soluble receptors of cytokines are able to modify cytokine activities and therefore the immune system, and some have intrinsic biological activities without mediation from their cytokines. The soluble interferon beta (IFN-ß) receptor is generated through alternative splicing of IFNAR2 and has both agonist and antagonist properties for IFN-ß, but its role is unknown. We previously demonstrated that a recombinant human soluble IFN-ß receptor showed intrinsic therapeutic efficacy in a mouse model of multiple sclerosis. Here we evaluate the potential biological activities of recombinant sIFNAR2 without the mediation of IFN-ß in human cells. Recombinant sIFNAR2 down-regulated the production of IL-17 and IFN-ɣ and reduced the cell proliferation rate. Moreover, it showed a strong antiviral activity, fully protecting the cell monolayer after being infected by the virus. Specific inhibitors completely abrogated the antiviral activity of IFN-ß, but not that of the recombinant sIFNAR2, and there was no activation of the JAK-STAT signaling pathway. Consequently, r-sIFNAR2 exerts immunomodulatory, antiproliferative and antiviral activities without IFN-ß mediation, and could be a promising treatment against viral infections and immune-mediated diseases.
PB MDPI
YR 2020
FD 2020
LK https://hdl.handle.net/10630/33636
UL https://hdl.handle.net/10630/33636
LA eng
NO Hurtado-Guerrero, I.; Hernáez, B.; Pinto-Medel, M.J.; Calonge, E.; Rodriguez-Bada, J.L.; Urbaneja, P.; Alonso, A.; Mena-Vázquez, N.; Aliaga, P.; Issazadeh-Navikas, S.; et al. Antiviral, Immunomodulatory and Antiproliferative Activities of Recombinant Soluble IFNAR2 without IFN-ß Mediation. J. Clin. Med. 2020, 9, 959. https://doi.org/10.3390/jcm9040959
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 21 ene 2026