RT Journal Article
T1 Large-Scale Dendrimer-Based Uneven Nanopatterns for the Study of Local RGD Density Effects on Cell Adhesion
A1 Lagunas, Anna
A1 Castaño, Albert G
A1 Artés, Juan M
A1 Vida-Pol, Yolanda
A1 Collado-Martín, Daniel
A1 Pérez-Inestrosa, Ezequiel
A1 Gorostiza, Pau
A1 Claros-Gil, Silvia
A1 Andrades-Gómez, José Antonio
A1 Samitier, Josep
K1 Dendrímeros
AB Cell adhesion processes are governed by the nanoscale arrangement of the extracellular matrix (ECM), being more affected by local rather than global concentrations of cell adhesive ligands. In many cell-based studies, grafting of dendrimers on surfaces has shown the benefits of the local increase in concentration provided by the dendritic configuration, although the lack of anyreported surface characterization has limited any direct correlation between dendrimer disposition and cell response. In order to establish a proper correlation, some control over dendrimer surface deposition is desirable. Here, dendrimer nanopatterning has been employed to address arginine–glycine–aspartic acid (RGD) density effects on cell adhesion. Nanopatterned surfaces were fully characterized by atomic force microscopy (AFM), scanning tunneling microscopy (STM) and X-ray photoelectron spectroscopy (XPS), showing that tunable distributions of cell adhesive ligands on the surface are obtained as a functionof the initial dendrimer bulk concentration. Cell experiments showed a clear correlation with dendrimer surface layout: Substrates presenting regions of high local ligand density resulted in a higher percentage of adhered cells and a higherdegree of maturation of focal adhesions (FAs). Therefore, dendrimer nanopatterning is presented as a suitable and controlled approach to address the effect of local ligand density on cell response. Moreover, due to the easy modification of dendrimer peripheral groups, dendrimer nanopatterning can be further extended to other ECM ligands having density effects on cells.
YR 2014
FD 2014
LK https://hdl.handle.net/10630/33415
UL https://hdl.handle.net/10630/33415
LA eng
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 19 ene 2026