RT Journal Article
T1 Indexing the Pseudomonas specialized metabolome enabled the discovery of poaeamide B and the bananamides
A1 Nguyen, Don D.
A1 Melnik, Alexey V.
A1 Koyama, Nobuhiro
A1 Lu, Xiaowen
A1 Schorn, Michelle
A1 Fang, Jinshu
A1 Aguinaldo, Kristen
A1 Lincecum, Tommie L. Jr
A1 Ghequire, Maarten G. K.
A1 Carrion, Víctor J
A1 Cheng, Tina L.
A1 Duggan, Brendan M.
A1 Malone, Jacob G
A1 Mauchline, Tim H
A1 Sanchez, Laura M.
A1 Kilpatrick, A. Marm
A1 Raaijmakers, Jos M
A1 de Mot, René
A1 Mooree, Bradley S.
A1 Medema, Marnix
A1 Dorrestein, Pieter C.
K1 Pseudomonas
AB Pseudomonads are cosmopolitan microbes able to produce a wide array of specialized metabolites.These molecules allow Pseudomonas to scavenge nutrients, sense population density, and enhanceor inhibit growth of competing microbes. However, these valuable metabolites are typicallycharacterized one-molecule-one-microbe at a time instead of inventoried in large numbers. Toindex and map the diversity of molecules detected from these organisms, 260 strains ofecologically diverse origins were subjected to mass spectrometry-based molecular networking.Molecular networking not only enables dereplication of molecules, but also sheds light on theirstructural relationships. Moreover, it accelerates discovery of new molecules. Herein, throughindexing the Pseudomonas specialized metabolome, we report the molecular networking-baseddiscovery of four molecules and their evolutionary relationships: a poaeamide analog, and amolecular sub-family of cyclic lipopeptides, the bananamides 1, 2, and 3. Analysis of theirbiosynthetic gene cluster shows that it constitutes a distinct evolutionary branch of thePseudomonas cyclic lipopeptides. Through analysis of an additional 370 extracts of wheat associatedPseudomonas, we demonstrate how the detailed knowledge from our reference indexcan be efficiently propagated to annotate complex metabolomic data from other studies akin to theway newly generated genomic information can be compared to data from public databases.
PB Springer Nature
YR 2016
FD 2016-10-31
LK https://hdl.handle.net/10630/33952
UL https://hdl.handle.net/10630/33952
LA eng
NO Nguyen, D., Melnik, A., Koyama, N. et al. Indexing the Pseudomonas specialized metabolome enabled the discovery of poaeamide B and the bananamides. Nat Microbiol 2, 16197 (2017). https://doi.org/10.1038/nmicrobiol.2016.197
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026