RT Journal Article
T1 The G protein-coupled receptor heterodimer network (GPCR-HetNet) and its hub components
A1 Borroto-Escuela, Dasiel O.
A1 Brito, Ismel
A1 Romero-Fernandez, Wilber
A1 Di Palma, Michael
A1 Oflijan, Julia
A1 Skieterska, Kamila
A1 Duchou, Jolien
A1 Van Craenenbroeck, Kathleen
A1 Suárez-Boomgaard, Diana
A1 Rivera, Alicia
A1 Guidolin, Diego
A1 Agnati, Luigi F.
A1 Fuxe, Kjell
K1 Receptores
AB G protein-coupled receptors (GPCRs) oligomerization has emerged as a vital characteristic of receptor structure. Substantial experimental evidence supports the existence of GPCR-GPCR interactions in a coordinated and cooperative manner. However, despite the current development of experimental techniques for large-scale detection of GPCR heteromers, in order to understand their connectivity it is necessary to develop novel tools to study the global heteroreceptor networks. To provide insight into the overall topology of the GPCR heteromers and identify key players, a collective interaction network was constructed. Experimental interaction data for each of the individual human GPCR protomers was obtained manually from the STRING and SCOPUS databases. The interaction data were used to build and analyze the network using Cytoscape software. The network was treated as undirected throughout the study. It is comprised of 156 nodes, 260 edges and has a scale-free topology. Connectivity analysis reveals a significant dominance of intrafamily versus interfamily connections. Most of the receptors within the network are linked to each other by a small number of edges. DRD2, OPRM, ADRB2, AA2AR, AA1R, OPRK, OPRD and GHSR are identified as hubs. In a network representation 10 modules/clusters also appear as a highly interconnected group of nodes. Information on this GPCR network can improve our understanding of molecular integration. GPCR-HetNet has been implemented in Java and is freely available at http://www.iiia.csic.es/~ismel/GPCR-Nets/index.html.
PB MDPI
SN 16616596
YR 2014
FD 2014-05-14
LK https://hdl.handle.net/10630/45006
UL https://hdl.handle.net/10630/45006
LA eng
NO Borroto-Escuela, D.O.; Brito, I.; Romero-Fernandez, W.; Di Palma, M.; Oflijan, J.; Skieterska, K.; Duchou, J.; Van Craenenbroeck, K.; Suárez-Boomgaard, D.; Rivera, A.; et al. The G Protein-Coupled Receptor Heterodimer Network (GPCR-HetNet) and Its Hub Components. Int. J. Mol. Sci. 2014, 15, 8570-8590. https://doi.org/10.3390/ijms15058570
NO Swedish Royal Academy of Sciences
NO Karolinska Institutets Forskningsstiftelser
NO Swedish Medical Research Council (Swedish Research Council)
NO Fundació La Marató de TV3
NO Hjärnfonden
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 3 mar 2026