RT Conference Proceedings
T1 Positive biocompatibility of several graphene derivatives with dopaminergic cells at long term culture
A1 Rodríguez-Losada, Noela
A1 Wendelbo, Rune
A1 Arenas, Ernest
A1 Aguirre-Gómez, José Ángel
K1 Biomedicina
K1 Materiales nanoestructurados
AB Carbon nanomaterial graphene (G) can form a three-dimensional porous structure with efficient bioconjugation and cell differentiation properties, providing a promising scaffold for neural regeneration. Aims: To study this putative new application of G, we cultured a clonal substantia nigra dopaminergic neuronal progenitor cell line (SN4741) in presence of G as scaffold. Methods: Cells were cultured in DMEM/10% FCS to about 80% confluence and incubated with different concentrations (0.001 to 1 mg/ml) of three chemically different G derivatives (G oxide (GO); partially reduced GO (PRGO) and fully reduced GO (FRGO)) and two different presentation matrixes as powder and films. Cell viability was measured by the MTT assay. To study cellular characterization, morphology and assessment of cell engraftment into G films, we analyzed the immunostaining of the neuronal marker NeuN, the anti-rat Beta-3-tubulin antibody, and the anti-rabbit DCX as immature neuronal marker. Lactate dehydrogenase was measured in the culture supernatant. Results: We found similar increase of survival and metabolism (30-40%) at low concentrations of PRGO and FRGO (0.05-0.01 mg/ml) compared with the higher concentration (1 mg/ml), no changes were seen in the GO group. PRGO or FRGO films showed an increased in the effective anchorage capacity to nest into the G matrix and in the maturation of the dopaminergic SN4741 cells. Conclusions: G scaffolds could offer a powerful platform for neural stem cells, direct cell conversion techniques and neural tissue engineering.
YR 2015
FD 2015-09-23
LK http://hdl.handle.net/10630/10291
UL http://hdl.handle.net/10630/10291
LA eng
NO Este trabajo presentado inicialmente como contribucion en poster fue seleccionada por el comite organizador por su calidad e invitada para ser presentada como comunicación oral
NO This work has been supported by the University of Malaga. Campus de Excelencia Internacional Andalucia Tech, and the Norwegian Research Council (grant nº 215086)
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026