RT Journal Article
T1 Pigment epithelium-derived factor (PEDF) and PEDF-receptor in the adult mouse brain: Differential spatial/temporal localization pattern
A1 De-Diego-Otero, María Yolanda
A1 Giráldez-Pérez, Rosa María
A1 Lima-Cabello, Elena
A1 Heredia-Farfán, Raúl
A1 Calvo Medina, Rocío
A1 Sánchez-Salido, Lourdes
A1 Pérez-Costillas, Lucía María
K1 Ratones
K1 Cerebro
K1 Epitelio
AB Pigment epithelium-derived factor (PEDF) is a multifunctional protein which was initially described in the retina, although it is also present in other tissues. It functions as an antioxidant agent promoting neuronal survival. Recently, a PEDF receptor has shown an elevated binding affinity for PEDF. There are no relevant data regarding the distribution of both proteins in the brain, therefore the main goal of this work was to investigate the spatiotemporal presence of PEDF and PEDFR in the adult mouse brain, and to determine the PEDF blood level in mouse and human. The localization of both proteins was analyzed by different experimental methods such as immunohistochemistry, western-blotting, and also by enzyme-linked immunosorbent assay. Differential expression was found in some telencephalic structures and positive signals for both proteins were detected in the cerebellum. The magnitude of the PEDFR labeling pattern was higher than PEDF and included some cortical and subventricular areas. Age-dependent changes in intensity of both protein immunoreactions were found in the cortical and hippocampal areas with greater reactivity between 4 and 8 months of age, whilst others, like the subventricular zones, these differences were more evident for PEDFR. Although ubiquitous presence was not found in the brain for these two proteins, their relevant functions must not be underestimated. It has been described that PEDF plays an important role in neuroprotection and data provided in the present work represents the first extensive study to understand the relevance of these two proteins in specific brain areas.
YR 2020
FD 2020-05-19
LK https://hdl.handle.net/10630/29951
UL https://hdl.handle.net/10630/29951
LA eng
NO de Diego‐Otero, Y., Giráldez‐Pérez, R. M., Lima‐Cabello, E., Heredia‐Farfan, R., Calvo Medina, R., Sanchez‐Salido, L., & Pérez Costillas, L. (2021). Pigment epithelium‐derived factor ( <scp>PEDF</scp> ) and <scp>PEDF‐receptor</scp> in the adult mouse brain: Differential spatial/temporal localization pattern. Journal of Comparative Neurology, 529(1), 141–158.
NO The authors wish to thank D.W.E. Ramsden for the manuscript revision. Microscopy Service of the Central Research Support Services of the University of Málaga (SCAI). Y. D. D. O. is under a contract from the “Nicolas Monardes” programme of the “Servicio Andaluz de Salud,” Regional Ministry of Health of the Andalusian Government, Andalusia, Spain. Partial funds from FEDER-EU of the European Regional Development Funds program. Grant Sponsors: Spanish Ministry of Science and Innovation; Grant numbers: SAF2008-00486. Health Ministry of the Andalusian Regional Government; Grant number: PI2009-0507. Innovation and Science Ministry of the Andalusian Regional Government; Grant number: P10-CTS-05704 and CTS546. Foundation Jerome Lejeune (Paris, France). Fundación Alicia Koplowitz (Madrid. Spain).
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026