RT Journal Article
T1 Ammonium regulates the development of pine roots through hormonal crosstalk and differential expression of transcription factors in the apex
A1 Ortigosa Peña, Francisco
A1 Lobato-Fernández, César
A1 Shikano, Hitomi
A1 Ávila-Sáez, Concepción
A1 Taira, Shu
A1 Cánovas-Ramos, Francisco Miguel
A1 Cañas-Pendón, Rafael Antonio
K1 Plantas - Nutrición
K1 Coniferas
AB Ammonium is a prominent source of inorganic nitrogen for plant nutrition, but excessive amounts can be toxic for many species. However, most conifers are tolerant to ammonium, a relevant physiological feature of this ancient evolutionary lineage. For a better understanding of the molecular basis of this trait, ammonium‐induced changes in the transcriptome of maritime pine (Pinus pinaster Ait.) root apex have been determined by laser capture microdissection and RNA sequencing. Ammonium promoted changes in the transcriptional profiles of multiple transcription factors, such as SHORT‐ROOT, and phytohormone‐related transcripts, such as ACO, involved in the development of the root meristem. Nano‐PALDI‐MSI and transcriptomic analyses showed that the distributions of IAA and CKs were altered in the root apex in response to ammonium nutrition. Taken together, the data suggest that this early response is involved in the increased lateral root branching and principal root growth, which characterize the long‐term response to ammonium supply in pine. All these results suggest that ammonium induces changes in the root system architecture through the IAA‐CK‐ET phytohormone crosstalk and transcriptional regulation.
PB Wiley
YR 2021
FD 2021-11-01
LK https://hdl.handle.net/10630/23242
UL https://hdl.handle.net/10630/23242
LA eng
NO Ortigosa, F., Lobato‐Fernández, C., Shikano, H., Ávila, C., Taira, S., Cánovas, F.M. et al. (2021) Ammonium regulates the development of pine roots through hormonal crosstalk and differential expression of transcription factors in the apex. Plant, Cell & Environment, 1–21. https://doi.org/10.1111/pce.14214
NO This study was funded by Spanish Ministerio de Ciencia e Innovación, grant numbers BIO2015‐73512‐JIN MINECO/AEI/FEDER, UE; RTI2018‐094041‐B‐I00 and EQC2018‐004346‐P. Funding for open access charge: Universidad de Málaga/CBUA. FO was supported by grants from the Universidad de Málaga (Programa Operativo de Empleo Juvenil vía SNJG, UMAJI11, FEDER, FSE, Junta de Andalucía) and BIO‐114, Junta de Andalucía
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 21 ene 2026