RT Journal Article
T1 The Role of Phospholipase D and MAPK Signaling Cascades in the Adaption of Lichen Microalgae to Desiccation: Changes in Membrane Lipids and Phosphoproteome.
A1 Gasulla, Francisco
A1 Barreno Rodríguez, Eva
A1 López Parages, María
A1 Cámara, J.
A1 Jiménez-Gámez, Carlos
A1 Dörmann, Peter
A1 Bartels, Dorothea
K1 Líquenes - Aspectos moleculares
AB Classically, lichen phycobionts are described as poikilohydricorganisms able to undergo desiccation due to the constitutivepresence of molecular protection mechanisms.However, little is known about the induction of cellular responsesin lichen phycobionts during drying. The analysis ofthe lipid composition of the desiccated lichen microalgaAsterochloris erici revealed the unusual accumulation ofhighly polar lipids (oligogalactolipids and phosphatidylinositol),which prevents the fusion of membranes during stress,but also the active degradation of cone-shaped lipids (monogalactosyldiacylglyceroland phosphatidylethanolamine) tostabilize membranes in desiccated cells. The level of phosphatidicacid increased 7-fold during desiccation, implicatinga possible role for phospholipase D (PLD) in the responseto osmotic stress. Inhibition of PLD with 1-butanol markedlyimpaired the recovery of photosynthesis activity in A. ericiupon desiccation and salt stress (2M NaCl). These twohyperosmotic stresses caused the phosphorylation of c-JunN-terminal kinase (JNK) and p38-like mitogen-activatedprotein kinase (MAPK) and the dephosphorylation of extracellularsignal-regulated kinase (ERK). The incubation with1-butanol reduced the phosphorylation of JNK-like proteinsand increased the dephosphorylation of ERK-like proteins,which indicates an upstream control of MAPK cascades byPLD. The phosphoproteome showed that desiccation causedthe phosphorylation of several proteins in A. erici, most ofthem involved in protein turnover. The results demonstratethat lichen phycobionts possess both constitutive and inducibleprotective mechanisms to acquire desiccation tolerance.Among others, these responses are controlled by thePLD pathway through the activation of MAPK cascades.
PB Oxford University Press
YR 2016
FD 2016
LK https://hdl.handle.net/10630/36975
UL https://hdl.handle.net/10630/36975
LA eng
NO Gasulla F, Barreno E, Parages ML, Cámara J, Jiménez C, Dörmann P, Bartels D. The Role of Phospholipase D and MAPK Signaling Cascades in the Adaption of Lichen Microalgae to Desiccation: Changes in Membrane Lipids and Phosphoproteome. Plant Cell Physiol. 2016 Sep;57(9):1908-20. doi: 10.1093/pcp/pcw111. Epub 2016 Jun 22. PMID: 27335354
NO https://academic.oup.com/pages/self_archiving_policy_b
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 23 ene 2026