RT Journal Article
T1 In vitro and in vivo effects of ulvan polysaccharides from Ulva rigida
A1 García-Márquez, Jorge
A1 Rodrigues Moreira, Bruna
A1 Valverde-Guillén, Piedad
A1 Latorre-Redoli, Sofía
A1 Caneda-Santiago, Candela T.
A1 Acién-Fernández, Francisco Gabriel
A1 Martínez-Manzanares, Eduardo
A1 Mari-Beffa, Manuel
A1 Abdala-Díaz, Roberto Teófilo
K1 Compuestos bioactivos vegetales
K1 Antineoplásicos
K1 Algas-Biotecnología
AB One of the main bioactive compounds of interest from the Ulva species is the sulfated polysaccharide ulvan, which has recently attracted attention for its anticancer properties. This study investigated the cytotoxic activity of ulvan polysaccharides obtained from Ulva rigida in the following scenarios: (i) in vitro against healthy and carcinogenic cell lines (1064sk (human fibroblasts), HACAT (immortalized human keratinocytes), U-937 (a human leukemia cell line), G-361 (a human malignant melanoma), and HCT-116 (a colon cancer cell line)) and (ii) in vivo against zebrafish embryos. Ulvan exhibited cytotoxic effects on the three human cancer cell lines tested. However, only HCT-116 demonstrated sufficient sensitivity to this ulvan to make it relevant as a potential anticancer treatment, presenting an LC50 of 0.1 mg mL−1. The in vivo assay on the zebrafish embryos showed a linear relationship between the polysaccharide concentration and growth retardation at 7.8 hpf mL mg−1, with an LC50 of about 5.2 mg mL−1 at 48 hpf. At concentrations near the LC50, toxic effects, such as pericardial edema or chorion lysis, could be found in the experimental larvae. Our in vitro study supports the potential use of polysaccharides extracted from U. rigida as candidates for treating human colon cancer. However, the in vivo assay on zebrafish indicated that the potential use of ulvan as a promising, safe compound should be limited to specific concentrations below 0.001 mg mL−1 since it revealed side effects on the embryonic growth rate and osmolar balance.
PB MDPI
YR 2023
FD 2023-04-28
LK https://hdl.handle.net/10630/26991
UL https://hdl.handle.net/10630/26991
LA eng
NO García-Márquez J, Moreira BR, Valverde-Guillén P, Latorre-Redoli S, Caneda-Santiago CT, Acién G, Martínez-Manzanares E, Marí-Beffa M, Abdala-Díaz RT. In Vitro and In Vivo Effects of Ulvan Polysaccharides from Ulva rigida. Pharmaceuticals. 2023; 16(5):660. https://doi.org/10.3390/ph16050660
NO Partial funding for open access charge: Universidad de Málaga
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 19 ene 2026