RT Journal Article
T1 Galanin receptor-1 modulates 5-hydroxtryptamine-1A signaling via heterodimerization
A1 Borroto Escuela, Dasiel Óscar
A1 Narváez-Peláez, Manuel
A1 Marcellino, Daniel
A1 Parrado-Romero, Concepción
A1 Narváez-Bueno, José Ángel
A1 Tarakanov, Alexander O.
A1 Agnati, Luigi F.
A1 Díaz-Cabiale, Zaida
A1 Fuxe, Kjell
A1 Díaz-Cabiale, Zaida
K1 Neurorreceptores
K1 Neuropéptidos
AB Previous biochemical, cardiovascular and behavioral work has given evidence for the existence of antagonistic galanin receptor-5-HT1A receptor interactions in the brain. In this study we investigated the existence of GalR1-5-HT1A receptor heteromers and their functional characteristics. In mammalian cells transfected with GFP2-tagged 5-HT1A receptor and YFP-tagged GalR1 receptor, a proximity-based fluorescence resonance energy transfer technique was used and it has been demonstrated that GalR1-5-HT1A receptors heteromerize. Furthermore, signaling by either the mitogen-activated protein kinase (MAPK) or adenylyl cyclase (AC) pathways by these heteromers indicates a trans-inhibition phenomenon through their interacting interface via allosteric mechanisms that block the development of an excessive activation of Gi/o and an exaggerated inhibition of AC or stimulation of MAPK activity. The presence of these heteromers in the discrete brain regions is postulated based on the existence of GalR-5-HT1A receptor-receptor interactions previously described in the brain and gives rise to explore possible novel therapeutic strategies for treatment of depression by targeting the GalR1-5-HT1A heteromers.
PB Elsevier
SN 10902104
YR 2010
FD 2010-03-19
LK https://hdl.handle.net/10630/45020
UL https://hdl.handle.net/10630/45020
LA eng
NO Dasiel O. Borroto-Escuela, Manuel Narvaez, Daniel Marcellino, Concepción Parrado, José Angel Narvaez, Alexander O. Tarakanov, Luigi F. Agnati, Zaida Díaz-Cabiale, Kjell Fuxe, Galanin receptor-1 modulates 5-hydroxtryptamine-1A signaling via heterodimerization, Biochemical and Biophysical Research Communications, Volume 393, Issue 4, 2010, Pages 767-772, ISSN 0006-291X, https://doi.org/10.1016/j.bbrc.2010.02.078. (https://www.sciencedirect.com/science/article/pii/S0006291X10002986)
NO Swedish Medical Research Council
NO Torsten and Ragnar Söderberg Foundation
NO Hjärnfonden
NO Marianne and Marcus Wallenberg Foundation
NO Fundació La Marató de TV3
NO Generalitat de Catalunya
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 4 mar 2026