RT Conference Proceedings
T1 Unraveling the importance of vesicle trafficking for the movement of geminiviruses
T2 Dissecting the relevance of vesicle trafficking for the movement of geminiviruses
A1 Cana Quijada, José Francisco
A1 Morales Martínez, Pablo
A1 Rosas-Díaz, Tábata Victoria
A1 Navarro, José Antonio
A1 Pallás, Vicente
A1 Lozano-Durán, Rosa
A1 Castillo-Garriga, Araceli
A1 Rodríguez-Bejarano, Eduardo
K1 Virus fitopatógenos
K1 Virus fitopatógenos
K1 Proteínas vegetales
K1 Tomates - Enfermedades y plagas
K1 Tomates - Variedades
AB Tomato yellow leaf curl disease is one of the most important threats to tomato crops worldwide. One of itscausal agents, Tomato yellow leaf curl Sardinian virus (TYLCSV), is a monopartite member of the genusBegomovirus from the family Geminiviridae. Due to the few proteins encoded by their viral genome,geminiviruses rely heavily on host cellular machinery and interact with a wide range of plant proteins tocomplete all processes required for infection, such as viral replication, movement and suppression or evasionof plant defense mechanisms. Therefore, identifying the host proteins involved in viral infection will be anessential step towards understanding the mechanisms underlying this process.Using a reverse genetic approach our group identified a series of genes involved in vesicle trafficking, whichaffect geminivirus infection. Four of them are essential as their silencing produce a complete abolishment ofTYLCSV infection ( δ-COP, ARF1, CHC1, and CHC2). However, these genes do not affect TYLCSV replication. Aseries of experiments using confocal microscopy and viral proteins bound to fluorescent markers have beencarried out to determine the effect of inhibiting vesicle trafficking over the subcellular localization of TYLCSV’smovement proteins. The biological relevance of vesicle trafficking during geminivirus infection will bepresented and discussed.
YR 2022
FD 2022
LK https://hdl.handle.net/10630/25273
UL https://hdl.handle.net/10630/25273
LA spa
NO Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026