RT Conference Proceedings
T1 Sexual differences in hippocampal microglia of adult mice subjected to maternal separation stress.
A1 Muñoz-Martín, José
A1 Infantes-López, M. Inmaculada
A1 Chaves-Peña, Patricia
A1 Nieto-Quero, Andrea
A1 Zambrana-Infantes, Emma
A1 Pedraza-Benítez, María del Carmen
A1 Pérez-Martín, Margarita
K1 Estrés (Fisiología)
K1 Neurobiología del desarrollo - Diferencias sexuales
AB Introduction: It is well known that early life adversities could a"ect brain development andincrease the vulnerability to stress-related disorders later in adulthood. Nevertheless, the neurobiological mechanisms underlying this susceptibility have been poorly characterized and sex could be an important variable. Recently, microglia, which is involved in many neurodevelopmental processes such as neurogenesis and synaptic plasticity, has been proposed as a mediator of this stress response and early life stress could “prime” microglia to be over- responsive in future challenges. Objective: The analysis of hippocampal microglia morphology and distribution in the dentate gyrus (DG) of mice subjected to early stress. Methods: Female and male C57BL/6J mice were subjected to 3h daily maternal separation (MS) for 21 days. In postnatal day 60, adult mice undertook a single 2h restriction stress (RS). Accordingly, the experimental groups were as follows: CTRL, RS, MS, MS+RS. The DG was analyzed using immunohistochemistry techniques against Iba1 (microglia) following image analysis (ImageJ) to obtain morphological and distribution data of microglial somas and DG surface area. Results: Smaller DG surface area was observed in MS male mice compared with the CTRL group, but not in female. Furthermore, microglial soma area changed in a sex-dependent manner, having female mice from MS group an increased soma area than those of MS male mice. This was also observed to be region-specific, with a larger microglia soma in DG subgranular zone (SGZ) of MS female compared to MS male. Since microglia in this DG zone is involved in neurogenesis, this might suggest a possible change in the formation of new born neurons. Conclusion: These results revealed a di"erent microglial response to stress depending on the animal sex and open the door to a better understanding of neurobiological basis in pathologies like depression. .
PB IBRO Congress 2023
YR 2023
FD 2023
LK https://hdl.handle.net/10630/27687
UL https://hdl.handle.net/10630/27687
LA eng
NO Munoz-Martin, J., Infantes-López, M.I., Chaves-Peña, P., Nieto-Quero, A., Zambrana-Infantes, E., Pedraza, C., Pérez-Martín, M.  Sexual differences in hippocampal microglia of adult mice subjected to maternal separation stress (Poster). IBRO 2023, Granada, Spain.
NO University of Málaga, the project PID2020-117464RB-I00 from Ministerio de Ciencia e Innovación (MCIN/AEI) Spain, awarded to Pedraza, C. and Pérez-Martín, M. ; the project P20_00460 from Consejería de Conocimiento, Investigación y Universidades, Junta de Andalucía awarded to Pedraza, C. and predoctoral fellowship FPU21/01318 awarded to Munoz- Martin, J. funded by MCIN/AEI, Spain.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026