RT Journal Article
T1 SlNRT1.5 transporter and the SlSKOR K+ channel jointly contribute to K+ translocation in tomato plants
A1 Martínez-Martínez, Almudena
A1 Botella, María Ángeles
A1 García-Legaz, Manuel Francisco
A1 López-Gómez, Elvira
A1 Amo, Jesús
A1 Rubio-Valverde, Lourdes
A1 Fernández-García, José Antonio
A1 Martínez, Vicente
A1 Rubio, Francisco
A1 Nieves-Cordones, Manuel
K1 Tomates
K1 Plantas
AB Accumulation of K+ in shoots is largely dependent on K+ transport via the xylem and has important implicationsnot only for K+ nutrition but also for stress tolerance. In tomato plants, the K+ channel SlSKOR contributed to K+translocation but the decrease in the shoot K+ content in slskor mutants was only ~15 %, indicating thatadditional K+ transport systems operated in the tomato stele. Here, we studied the physiological roles of thetransporter SlNRT1.5 in tomato plants, whose homolog in Arabidopsis, AtNRT1.5, contributed to xylem K+ load.By using heterologous expression of SlNRT1.5 in Xenopus oocytes and a slnrt1.5 knock-out mutant, we havegained insights into its role in shoot K+ nutrition. Expression of SlNRT1.5 in Xenopus oocytes resulted in K+efflux, similar to that mediated by AtNRT1.5, which could indicate that SlNRT1.5 operates as a K+ transportsystem. Plants lacking slnrt1.5 accumulated less K+ in shoots than WT plants under low external pH (4.5), andlow supply of K+ (0.05 mM) and N (0.5 mM). Interestingly, slnrt1.5 plants accumulated less Na+ and Cl- in shootsthan WT plants. Further analyses on slskor slnrt1.5 double mutant plants revealed an overlapping role of SlSKORand SlNRT1.5 in shoot K+ accumulation. Double mutants showed a 40 % decrease in shoot K+ content incomparison with slskor and slnrt1.5 single mutants. Altogether, this study showed that SlNRT1.5 and SlSKOR aremajor players in shoot K+ accumulation in tomato plants.
PB Elsevier
YR 2024
FD 2024
LK https://hdl.handle.net/10630/40174
UL https://hdl.handle.net/10630/40174
LA eng
NO Martínez-Martínez et al., 2024. SlNRT1.5 transporter and the SlSKOR K+ channel jointly contribute to K+ translocation in tomato plants. Plant Stress 14. 100689
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 21 ene 2026