RT Conference Proceedings
T1 Effects of stimulation of cuneiform nucleus and the dorsomedial Hypothalamic nucleus and Perifornical area on the mechanisms involved in the control of laryngeal activity and subglottic pressure in spontaneously breathing anaesthetized rats.
A1 Carrillo-Franco, Laura
A1 González-García, Marta
A1 Díaz-Casares, Amelia
A1 Morales-Luque, Carmen
A1 López-González, Manuel Víctor
A1 Dawid-Milner, Marc Stefan
K1 Fisiología comparada
AB The dorsomedial Hypothalamic nucleus and Perifornical area (DMH-PeF) and the mesencephalic Cuneiform nucleus (CnF) have been involved in sympathetic activity due their connectivity with several nuclei involved in cardiorespiratory control. In previous studies we have demonstrated a functional interaction between hypothalamic and mesencephalic structures (DMH-PeF, dlPAG) with several pontine regions (PBc, A5) (2, 3). We have also shown that rostral and ventral pontine structures are involved in the changes of laryngeal caliber (4). The aim of this study was to characterize the relations between hypothalamic and mesencephalic regions involved in cardiorespiratory control and their possible role in modulating laryngeal activity and their possible effects on vocalization. Experimental studies were carried out with non-inbred male rats (n=42), Sprague-Dawley (250-300g). Animals were anesthetized with sodium pentobarbitone (60 mg/kg i.p., initial dose, supplemented 2mg/kg, i.v., as necessary). A double tracheal cannulation was used to obtain an “isolated glottis in situ” and to record respiratory airflow. Bilateral parietostomy allowed access to the DMH-PeF and CnF. Electrical (n=14) and chemical (n=14) stimulations of these regions using bipolar electrodes or glutamate was performed. Microinjections (n=14) of PBS-Evans Blue served as control purpose. Respiratory flow, pleural pressure, blood pressure and heart rate were also recorded.DMH-PeF and CnF PBS-Evans Blue microinjections did not produce any significant changes in any of the cardiorespiratory variables recorded. Glutamate microinjections within the DMH-PeF and CnF evoked a decrease of laryngeal resistance (subglottal pressure) (p<0,01 and p<0,001 respectively) with an inspiratory facilitatory response consisted of an increase in respiratory rate (p<0,001 in both cases), together with a pressor (p<0,001 and p<0,01 respectively) and tachycardic response (p<0,001in both cases)
YR 2023
FD 2023-07
LK https://hdl.handle.net/10630/27392
UL https://hdl.handle.net/10630/27392
LA eng
NO Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 19 ene 2026