RT Journal Article
T1 TTL Proteins Scaffold Brassinosteroid Signaling Components at the Plasma Membrane to Optimize Signal Transduction in Arabidopsis
A1 Amorim-Silva, Vitor
A1 García-Moreno, Álvaro
A1 Castillo-Garriga, Araceli
A1 Lakhssassi, Naoufal
A1 Esteban del Valle, Alicia
A1 Pérez-Sancho, Jessica
A1 Li, Yansha
A1 Posé-Padilla, David
A1 Pérez-Rodríguez, Josefa
A1 Lin, Jinxing
A1 Valpuesta-Fernández, Victoriano
A1 Borsani, Omar
A1 Zipfel, Cyril
A1 Macho, Alberto P.
A1 Botella-Mesa, Miguel Ángel
K1 Hormonas vegetales
AB Brassinosteroids (BRs) form a group of steroidal hormones essential for plant growth, development, and stress responses. BRs are perceived extracellularly by plasma membrane receptor-like kinases that activate an interconnected signal transduction cascade, leading to the transcriptional regulation of BR-responsive genes. TETRATRICOPEPTIDE THIOREDOXIN-LIKE (TTL) genes are specific for land plants, and their encoded proteins are defined by the presence of protein–protein interaction motives, that is, an intrinsic disordered region at the N terminus, six tetratricopeptide repeat domains, and a C terminus with homology to thioredoxins. TTL proteins thus likely mediate the assembly of multiprotein complexes. Phenotypic, molecular, and genetic analyses show that TTL proteins are positive regulators of BR signaling in Arabidopsis (Arabidopsis thaliana). TTL3 directly interacts with a constitutively active BRASSINOSTEROID INSENSITIVE1 (BRI1) receptor kinase, BRI1-SUPPRESSOR1 phosphatase, and the BRASSINAZOLE RESISTANT1 transcription factor and associates with BR-SIGNALING KINASE1, BRASSINOSTEROID INSENSITIVE2 kinases, but not with BRI1-ASSOCIATED KINASE1. A functional TTL3-green fluorescent protein (GFP) shows dual cytoplasmic plasma membrane localization. Depleting the endogenous BR content reduces plasma membrane localization of TTL3-GFP, while increasing BR content causes its plasma membrane relocalization, where it strengthens the association of BR signaling components. Our results reveal that TTL proteins promote BR responses and suggest that TTL proteins may function as scaffold proteins by bringing together cytoplasmic and plasma membrane BR signaling components.
PB Oxford Academy
YR 2019
FD 2019-06-12
LK https://hdl.handle.net/10630/34682
UL https://hdl.handle.net/10630/34682
LA eng
NO Vítor Amorim-Silva, Álvaro García-Moreno, Araceli G. Castillo, Naoufal Lakhssassi, Alicia Esteban del Valle, Jessica Pérez-Sancho, Yansha Li, David Posé, Josefa Pérez-Rodriguez, Jinxing Lin, Victoriano Valpuesta, Omar Borsani, Cyril Zipfel, Alberto P. Macho, Miguel A. Botella, TTL Proteins Scaffold Brassinosteroid Signaling Components at the Plasma Membrane to Optimize Signal Transduction in Arabidopsis, The Plant Cell, Volume 31, Issue 8, August 2019, Pages 1807–1828, https://doi.org/10.1105/tpc.19.00150
NO This work was supported by the Ministerio de Economía y Competitividad (cofinanced by the European Regional Development Fund) (grant BIO2017-82609-R to M.A.B.) and by a Formación del Personal Investigador Fellowship from the Ministerio de Economía y Competitividad (FPI-BES 2015-071256 to A.G.-M.), by the Shanghai Center for Plant Stress Biology (Chinese Academy of Sciences), Chinese 1000 Talents Program (to A.P.M.), and by the Gatsby Charitable Foundation and the European Research Council (grant 309858 “PHOSPHinnATE” to C.Z.). Support was also provided by the Ramón y Cajal Program (RYC-2013-12699 MINECO-Universidad de Málaga, Spain to D.P.); by a fellowship from Agencia Española de Cooperación Internacional para el Desarrollo (Ministerio de Asuntos Exteriores y Cooperacion); and by the Ministerio de Educacion y Ciencia (Universidad de Málaga, Spain) to N.L.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 4 mar 2026