RT Journal Article
T1 Exploring the Developmental Potential of Human Germinal Vesicle Oocytes Aiming at Fertility Preservation: Can We Increase the Yields of Competent Oocytes through IVM Combined with Vitrification?
A1 Hao, Xia
A1 Phoon, Jessie
A1 Barbunopulos, Lina
A1 Sheikhi, Mona
A1 Reyes Palomares, Arturo
A1 Rodriguez-Wallberg, Kenny
K1 Reproducción humana asistida
K1 Fecundación in vitro
AB The rescue in vitro maturation (rIVM) of germinal vesicle oocytes (GVs) has been proposed to improve the total number of mature oocytes in women undergoing fertility preservation. Currently, there is no consensus about the clinical utility of this practice, and heterogeneity in the protocols used may influence the final outcomes. This study investigated the developmental potential of mature metaphase II (MII) human oocytes obtained from GVs after rIVM and the impact of applying vitrification at different timepoints either before or after rIVM. After randomization, oocytes were assigned to undergo rIVM and thereafter vitrification or intracytoplasmic sperm injection (ICSI), or to undergo direct vitrification-warming and thereafter rIVM and ICSI. The likelihood of obtaining MII oocytes was just slightly higher in the fresh rIVM group compared to the vitrification-warming-rIVM group. When comparing fresh rIVM that underwent subsequently ICSI, the fertilization and developmental rates up to the blastocyst stage were seen to be reduced in both groups that underwent vitrification either before or after rIVM. Although some blastocysts were obtained in the fresh rIVM-ICSI group, the efficacy of these methods was low overall, suggesting that the further development of protocols for IVM conducted early after denudation is needed to improve the final results of rIVM aiming at fertility preservation.
PB MDPI
YR 2022
FD 2022-03-19
LK https://hdl.handle.net/10630/44689
UL https://hdl.handle.net/10630/44689
LA eng
NO Hao, X.; Phoon, J.; Barbunopulos, L.; Sheikhi, M.; Palomares, A.R.; Rodriguez-Wallberg, K.A. Exploring the Developmental Potential of Human Germinal Vesicle Oocytes Aiming at Fertility Preservation: Can We Increase the Yields of Competent Oocytes through IVM Combined with Vitrification? J. Clin. Med. 2022, 11, 1703. https://doi.org/10.3390/jcm11061703
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 3 mar 2026