RT Journal Article
T1 Localization of the cannabinoid CB1 receptor and the 2-AG synthesizing (DAGLα) and degrading (MAGL, FAAH) enzymes in cells expressing the Ca(2+)-binding proteins calbindin, calretinin, and parvalbumin in the adult rat hippocampus
A1 Rivera-González, Patricia
A1 Arrabal, Sergio
A1 Cifuentes-Rueda, Manuel
A1 Mateos-Grondona, Jesús
A1 Pérez-Martín, Margarita
A1 Rubio-Lamia, Leticia Olga
A1 Vargas, Antonio
A1 Serrano, Antonia
A1 Pavón, Francisco Javier
A1 Suárez-Pérez, Juan
A1 Rodriguez de Fonseca, Fernando
K1 Inmunoquímica
K1 Hipocampo (Cerebro)
K1 Microscopía confocal
K1 Proteínas transportadoras del calcio
K1 Modelos animales en investigación
AB The retrograde suppression of the synaptic transmission by the endocannabinoid sn-2-arachidonoylglycerol (2-AG) is mediated by the cannabinoid CB1 receptors and requires the elevation of intracellular Ca(2+) and the activation of specific 2-AG synthesizing (i.e., DAGLα) enzymes. However, the anatomical organization of the neuronal substrates that express 2-AG/CB1 signaling system-related molecules associated with selective Ca(2+)-binding proteins (CaBPs) is still unknown. For this purpose, we used double-label immunofluorescence and confocal laser scanning microscopy for the characterization of the expression of the 2-AG/CB1 signaling system (CB1 receptor, DAGLα, MAGL, and FAAH) and the CaBPs calbindin D28k, calretinin, and parvalbumin in the rat hippocampus. CB1, DAGLα, and MAGL labeling was mainly localized in fibers and neuropil, which were differentially organized depending on the hippocampal CaBPs-expressing cells. CB(+) 1 fiber terminals localized in all hippocampal principal cell layers were tightly attached to calbindin(+) cells (granular and pyramidal neurons), and calretinin(+) and parvalbumin(+) interneurons. DAGLα neuropil labeling was selectively found surrounding calbindin(+) principal cells in the dentate gyrus and CA1, and in the calretinin(+) and parvalbumin(+) interneurons in the pyramidal cell layers of the CA1/3 fields. MAGL(+) terminals were only observed around CA1 calbindin(+) pyramidal cells, CA1/3 calretinin(+) interneurons and CA3 parvalbumin(+) interneurons localized in the pyramidal cell layers. Interestingly, calbindin(+) pyramidal cells expressed FAAH specifically in the CA1 field. The identification of anatomically related-neuronal substrates that expressed 2-AG/CB1 signaling system and selective CaBPs should be considered when analyzing the cannabinoid signaling associated with hippocampal functions.
PB Frontiers
YR 2014
FD 2014-06-27
LK https://hdl.handle.net/10630/40141
UL https://hdl.handle.net/10630/40141
LA eng
NO Patricia Rivera, Sergio Arrabal, Manuel Cifuentes, Jesús M Grondona, Margarita Pérez-Martín, Leticia Rubio, Antonio Vargas, Antonia Serrano, Francisco J Pavón, Juan Suárez, Fernando Rodríguez de Fonseca. Localization of the cannabinoid CB1 receptor and the 2-AG synthesizing (DAGLα) and degrading (MAGL, FAAH) enzymes in cells expressing the Ca(2+)-binding proteins calbindin, calretinin, and parvalbumin in the adult rat hippocampus. Front Neuroanat . 2014 Jun 27:8:56. doi: 10.3389/fnana.2014.00056.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 19 ene 2026