RT Journal Article
T1 Concerted transport and phosphorylation of diacylglycerol at ER–PM contact sites regulate phospholipid dynamics during stress.
A1 García-Hernández, Selene
A1 Morello-López, Jorge
A1 Haslam, Richard
A1 Amorim-Silva, Vitor
A1 Moya-Cuevas, José
A1 Catalá, Rafael
A1 Michaelson, Louise
A1 Pérez-Sancho, Jessica
A1 Marković, Vedrana
A1 Salinas, Julio
A1 Napier, Johnathan
A1 Jaillais, Yvon
A1 Ruiz-López, Noemí
A1 Botella-Mesa, Miguel Ángel
K1 Transporte biológico
K1 Alcoholes
K1 Estrés (Fisiología)
AB A universal response of plants to environmental stresses is the activation of plasma membrane (PM) phospholipase C, which hydrolyzes phosphoinositides to produce soluble inositol phosphate and diacylglycerol (DAG). Because of their conical shape, DAG amounts have to be tightly regulated or they can destabilize membranes. We previously showed that upon stress, Synaptotagmin1 (SYT1) transports DAG from the PM to the endoplasmic reticulum (ER) at ER–PM Contact Sites (CS). Here, we addressed the fate of the incoming DAG in the ER. We show that diacylglycerol kinases (DGKs) DGK1 and DGK2 form a module with SYT1 functionally coupling DAG transport and phosphorylation at ER–PM CS. Although SYT1 and DGK1/DGK2 do not show exclusive ER–PM CS localization, their interaction occurs specifically at ER–PM CS and the removal of ER–PM CS abolishes the interaction. Lipidomic analysis of a dgk1dgk2 double mutant supports that DGK1 and DGK2 phosphorylate DAG at the ER and transcriptomic and phenotypic analyses indicate that SYT1 and DGK1/DGK2 are functionally related. Taken together, our results highlight a mechanism at ER–PM CS that coordinates the transfer of DAG from the PM to the ER by SYT1 upon stress and the concomitant phosphorylation of DAG by DGK1 and DGK2 at the ER. These findings underscore the critical role of spatial coordination in lipid metabolism during stress-induced membrane remodeling.
PB PNAS
YR 2025
FD 2025-06-02
LK https://hdl.handle.net/10630/38934
UL https://hdl.handle.net/10630/38934
LA eng
NO S. Garcia-Hernandez, J. Morello-López, R. Haslam, V. Amorim-Silva, J. Moya-Cuevas, R. Catalá, L. Michaelson, J. Pérez-Sancho, V. Marković, J. Salinas, J. Napier, Y. Jaillais, N. Ruiz-Lopez, & M.A. Botella, Concerted transport and phosphorylation of diacylglycerol at ER–PM contact sites regulate phospholipid dynamics during stress, Proc. Natl. Acad. Sci. U.S.A. 122 (23) e2421334122, https://doi.org/10.1073/pnas.2421334122 (2025).
NO Spanish Ministry for Science and Innovation
NO Junta de Andalucia
NO Consejería de Economía y Conocimiento de la Junta de Andalucía
NO European Research Council
NO European Molecular Biology Organization
NO BBSRC (UK) Institute Strategic Program Grant
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026