RT Journal Article
T1 Immunogene expression analysis in betanodavirus infected-Senegalese sole using an OpenArray® platform.
A1 Gémez-Mata, Juan
A1 Labella Vera, Alejandro Manuel
A1 Bandín Matos, Isabel
A1 Borrego-García, Juan José
A1 García-Rosado, Esther
K1 Peces-Virus
AB The transcriptomic response of Senegalese sole (Solea senegalensis) triggered by two betanodaviruses with different virulence to that fish species has been assessed using an OpenArray® platform based on TaqMan™ quantitative PCR. The transcription of 112 genes per sample has been evaluated at two sampling times in two organs (head kidney and eye/brain-pooled samples). Those genes were involved in several roles or pathways, such as viral recognition, regulation of type I (IFN-1)-dependent immune responses, JAK-STAT cascade, interferon stimulated genes, protein ubiquitination, virus responsive genes, complement system, inflammatory response, other immune system effectors, regulation of T-cell proliferation, and proteolysis and apoptosis. The highly virulent isolate, wSs160.3, a wild type reassortant containing a RGNNV-type RNA1 and a SJNNV-type RNA2 segments, induced the expression of a higher number of genes in both tested organs than the moderately virulent strain, a recombinant harbouring mutations in the protruding domain of the capsid protein. The number of differentially expressed genes was higher 2 days after the infection with the wild type isolate than at 3 days post-inoculation. The wild type isolate also elicited an exacerbated interferon 1 response, which, instead of protecting sole against the infection, increases the disease severity by the induction of apoptosis and inflammation-derived immunopathology, although inflammation seems to be modulated by the complement system. Furthermore, results derived from this study suggest a potential important role for some genes with high expression after infection with the highly virulent virus, such as rtp3, sacs and isg15. On the other hand, the infection with the mutant does not induce immune response, probably due to an altered recognition by the host, which is supported by a different viral recognition pathway, involving myd88 and tbkbp1.
PB Elsevier
YR 2021
FD 2021-03-30
LK https://hdl.handle.net/10630/23953
UL https://hdl.handle.net/10630/23953
LA eng
NO Gémez-Mata, Juan ; Labella, Alejandro ; Bandín, Isabel ; Borrego-Garcia, Juan Jose ; García-Rosado, Esther. Immunogene expression analysis in betanodavirus infected-Senegalese sole using an OpenArray® platform. Gene Volume 774, 30 March 2021, 145430. https://doi.org/10.1016/j.gene.2021.145430
NO Funding for open access charge: Universidad de Málaga / CBUA. This work has been supported by the projects AGL2014-54532-C2 and RTI2018-094687-B-C22 form the Spanish Government (Ministerio de Economía y Competitividad), co-funded by the FEDER.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026