RT Journal Article
T1 Antioxidant, Immunomodulatory and Potential Anticancer Capacity of Polysaccharides (Glucans) from Euglena gracilis G.A. Klebs
A1 Casas Arrojo, Virginia
A1 Arrojo-Agudo, María Ángeles
A1 Cárdenas García, Casimiro
A1 Carrillo Fernández, Paloma
A1 Pérez Manríquez, Claudia
A1 Martínez-Manzanares, Eduardo
A1 Abdala-Díaz, Roberto Teófilo
K1 Alimentos funcionales
AB The present study was carried out to determine the bioactivity of polysaccharides extracted from Euglena gracilis (EgPs). These were characterized by FT-IR and GC-MS. Cytotoxicity analyses (MTT) were performed on healthy human gingival fibroblast cell lines (HGF-1), obtaining an IC50 of 228.66 µg mL−1, and cell lines with anticancer activity for colon cancer (HCT-116), breast cancer (MCF-7), human leukemia (U-937, HL-60) and lung cancer (NCl-H460), showing that EgPs have anticancer activity, mainly in HTC-116 cells (IC50 = 26.1 µg mL−1). The immunological assay determined the immunomodulatory capacity of polysaccharides for the production of proinflammatory cytokines IL-6 and TNF-α in murine macrophages (RAW 264.7) and TNF-α in human monocytes (THP-1). It was observed that the EgPs had a stimulating capacity in the synthesis of these interleukins. The antioxidant capacity of polysaccharides and their biomass were analyzed using the ABTS method (18.30 ± 0.14% and (5.40 ± 0.56%, respectively, and the DPPH method for biomass (17.79 ± 0.57%). We quantitatively profiled HGF-1 proteins by liquid chromatography–tandem mass spectrometry analysis, coupled with 2-plex tandem mass tag labelling, in normal cells. In total, 1346 proteins were identified and quantified with high confidence, of which five were considered to be overexpressed. The data is available through ProteomeXchange, under identifier PXD029076.
PB IOAP-MDPI
YR 2022
FD 2022-11-10
LK https://hdl.handle.net/10630/25891
UL https://hdl.handle.net/10630/25891
LA eng
NO Casas-Arrojo V, Arrojo Agudo MdlÁ, Cárdenas García C, Carrillo P, Pérez Manríquez C, Martínez-Manzanares E, Abdala Díaz RT. Antioxidant, Immunomodulatory and Potential Anticancer Capacity of Polysaccharides (Glucans) from Euglena gracilis G.A. Klebs. Pharmaceuticals. 2022; 15(11):1379. https://doi.org/10.3390/ph15111379
NO Partial funding for open access charge: Universidad de Málaga
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 21 ene 2026