RT Journal Article
T1 Optimizing Extracellular Products from Vibrio proteolyticus for Their Use as Postbiotics in Aquaculture
A1 García-Márquez, Jorge
A1 Domínguez-Maqueda, Marta
A1 Pérez-Gómez, Olivia
A1 Cerezo, Isabel M.
A1 Espinosa Ruíz, Cristóbal
A1 Esteban, M. Ángeles
A1 Vallejo, Fernando
A1 Alarcón López, Francisco Javier
A1 Martínez Manzanares, Eduardo
A1 Tapia-Paniagua, Silvana Teresa
A1 Balebona-Accino, María del Carmen
A1 Moriñigo-Gutiérrez, Miguel Ángel
A1 Arijo-Andrade, Salvador
K1 Microbiología - Cultivos y medios de cultivo
K1 Prebióticos
AB Vibrio proteolyticus DCF12.2 has demonstrated its ability to be used as a probiotic for fish species. This study investigateshow different culture conditions influence the activity of its extracellular products (ECPs) in aquaculture, focusing onenzymatic and antibacterial activity, cytotoxicity, biofilm modulation, short-chain fatty acid (SCFA) profiles, and effects onPhotobacterium damselae subsp. piscicida virulence. Enzymatic assays showed a variety of hydrolytic activities, includ-ing amylase, caseinase, and collagenase, which can enhance digestion and nutrient absorption in fish. Antibacterial assaysrevealed that ECPs from V. proteolyticus grown in an experimental aquafeed and a partial replacement of that aquafeed by25% of a blend of microalgae inhibited P. damselae subsp. piscicida and P. damselae subsp. damselae. Cytotoxicity assaysindicated variable effects across fish cell lines, with increased viability in SAF-1 and DLB-1 cells under specific conditions,and decreased viability in PLHC-1 cells, suggesting potential antitumor properties. Biofilm assays showed that certainECP conditions reduced biofilm formation by Vibrio anguillarum, Aeromonas hydrophila, and Tenacibaculum maritimum.SCFA profiling detected acetic, iso-valeric, butyric, and valeric acids, which may contribute to antimicrobial activity andgut health. The ECPs significantly downregulated aip56 gene transcription, reducing the virulence of P. damselae subsp.piscicida. These findings suggest that ECPs from V. proteolyticus could be valuable aquafeed additives for enhancing fishnutrition, health, and disease resistance. Future research should aim to isolate and characterize the specific bioactive com-pounds responsible for these effects and elucidate their mechanisms of action for optimized application in aquaculture andother biotechnological fields.
PB Springer
YR 2025
FD 2025
LK https://hdl.handle.net/10630/40026
UL https://hdl.handle.net/10630/40026
LA eng
NO García-Márquez, J., Domínguez-Maqueda, M., Pérez-Gómez, O. et al. Optimizing Extracellular Products from Vibrio proteolyticus for Their Use as Postbiotics in Aquaculture. Mar Biotechnol 27, 120 (2025). https://doi.org/10.1007/s10126-025-10500-6
NO Universidad de Málaga/CBUA
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 19 ene 2026