RT Journal Article
T1 Phosphorylation of MAP Kinases crucially controls the response to environmental stress in Dunaliella viridis
A1 Ortiz Charneco, Guilermo
A1 López Parages, María
A1 Camarena Gómez, María Teresa
A1 Jiménez-Gámez, Carlos
K1 Microalgas - Aspectos ambientales
AB The green unicellular microalga Dunaliella viridis has the ability to cope with a wide variety of environmentalstressful conditions, such as thermal and osmotic shocks, high PAR, UV radiation and nitrogen deficiency. Thelack of a rigid cell wall makes D. viridis an excellent model organism to study stress signaling in eukaryoticunicellular organisms. Mitogen-activated protein kinases (MAPKs) are highly conserved serine/threonine kinasesthat convert extracellular stimuli into a wide range of responses at both cellular and nuclear levels. In eukaryoticcells, MAPKs are involved in both cell proliferation and differentiation (ERK pathway) and stress responses (JNKand p38 pathways), through protein kinase cascades. Significantly lesser phosphorylation levels of ERK-likeprotein were observed in D. viridis cultures acclimated to high salinity (3–4M NaCl). In contrast, JNK-like andp38-like proteins phosphorylation levels increased in stressed cells. Likewise, the efficacy of specific commercialinhibitors of the phosphorylation of ERK (PD98059), JNK (SP600125) and p38 (SB203580) revealed the importanceof JNK-like proteins in the maintenance of cell viability, the highlighted participation of p38-likeproteins and the non-direct implication of the ERK-like proteins in the acclimatization process. In summary,specific blockade of JNK- and p38-like cascades in stressed cells led to rapid cell death. The behavior of MAPKlikeproteins in algae is not known in depth, so the analysis of their mechanism of action, as well as their functionin this model microalga, will allow to estimate the fate of unicellular eukaryotic organisms in aquatic ecosystemssubjected to environmental stress derived from the conditions prevailing within a framework of global climatechange.
PB Elsevier
YR 2018
FD 2018-08-27
LK https://hdl.handle.net/10630/36969
UL https://hdl.handle.net/10630/36969
LA eng
NO Guillermo Ortiz Charneco, María L. Parages, M. Teresa Camarena-Gómez, Carlos Jiménez, Phosphorylation of MAP Kinases crucially controls the response to environmental stress in Dunaliella viridis, Environmental and Experimental Botany, Volume 156, 2018, Pages 203-213, ISSN 0098-8472, https://doi.org/10.1016/j.envexpbot.2018.08.030
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 21 ene 2026