RT Journal Article
T1 The synthetic molecule stauprimide impairs cell growth and migration in triple-negative breast cancer
A1 Carrillo Fernández, Paloma
A1 Bernal Muñoz, Manuel
A1 Téllez Quijorna, Clara
A1 Marrero Capitán, Ana Dácil
A1 Vidal-Valenzuela, Isabel
A1 Castilla, Laura
A1 Caro Salazar, Carlos
A1 Domínguez Moreno, Alejandro
A1 García-Martín, María Luisa
A1 Rodríguez-Quesada, Ana María
A1 Medina-Torres, Miguel Ángel
A1 Martínez-Póveda, Beatriz Amparo
K1 Mamas -- Cáncer
K1 Células madre
AB Stauprimide, a semi-synthetic derivative of staurosporine, is known mainly for its potent differentiation-enhancing properties in embryonic stem cells. Here, we studied the effects of stauprimide in cell growth and migration of triple-negative breast cancer cells in vitro, evaluating its potential antitumoral activity in an orthotopic mouse model of breast cancer in vivo. Our results from survival curves, EdU incorporation, cell cycle analysis and annexin-V detection in MDA-MB-231 cells indicated that stauprimide inhibited cell proliferation, arresting cell cycle in G2/M without induction of apoptosis. A decrease in the migratory capability of MDA-MB-231 was also assessed in response to stauprimide. In this work we pointed to a mechanism of action of stauprimide involving the modulation of ERK1/2, Akt and p38 MAPK signalling pathways, and the downregulation of MYC in MDA-MB-231 cells. In addition, orthotopic MDA-MB-231 xenograft and 4T1 syngeneic models suggested an effect of stauprimide in vivo, increasing the necrotic core of tumors and reducing metastasis in lung and liver of mice. Together, our results point to the promising role of stauprimide as a putative therapeutic agent in triple-negative breast cancer.
PB Elsevier
YR 2022
FD 2022-12-14
LK https://hdl.handle.net/10630/26491
UL https://hdl.handle.net/10630/26491
LA eng
NO Carrillo, P., Bernal, M., Téllez-Quijorna, C., Marrero, A. D., Vidal, I., Castilla, L., ... & Martínez-Poveda, B. (2023). The synthetic molecule stauprimide impairs cell growth and migration in triple-negative breast cancer. Biomedicine & Pharmacotherapy, 158, 114070.
NO MRI experiments were performed in the ICTS “NANBIOSIS”, more specifically in the U28 Unit at the Andalusian Centre for Nanomedicine & Biotechnology (BIONAND). Cell cultures were performed in the Cell Culture Service at the Central Support Services of Research (SCAI) of the University of Málaga.  //   Partial funding for open access charge: Universidad de Málaga / CBUA.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026