RT Conference Proceedings
T1 Detecting hydroxyl radical with a new two-photon fluorescent probe in living cells
A1 Nájera-Albendín, Francisco
A1 Benítez-Martín, Carlos
A1 Guadix-Domínguez, Juan Antonio
A1 Pérez-Pomares, José María
A1 Pérez-de-Inestrosa-Villatoro, Ezequiel
K1 Química orgániza - Congresos
AB Reactive oxygen species (ROS) are known to be involved in the onset and development of multiple diseases, including cancer, cardiovascular diseases, neurodegenerative diseases and diabetes among others. Generation of ROS is a phenomenon that results from normal cell metabolism as well as from the response to certain pathologic stimuli like certain cytokines, xenobiotics and bacterial infection. These ROS are highly reactive, short-lived molecules that play critical roles in the living cell and it is well accepted that cellular oxidative stress results from the imbalance between generation and elimination of ROS in cells. In this work, we describe the design and synthesis of a two photon (TP) organelle-targeting activatable fluorescent probe. This probe is a naphthalene-indoline compound that targets specifically lysosomes, reaching these organelles in the fluorescence “off” state and minimizing background reactions. Upon arrival at the lysosomes, the probe is triggered and a fluorescence “on” signal is observed that can be combined with TP microscopy to image the lysosomal •OH in living cells.
PB Real Sociedad Española de Química
YR 2018
FD 2018-06-20
LK https://hdl.handle.net/10630/16041
UL https://hdl.handle.net/10630/16041
LA eng
NO XXVII Biennial Meeting in Organic Chemistry
NO Real Sociedad Española de Química y Grupo Especializado de Química Orgánica de la RSEQ•Universidade de Santiago de Compostela • CIQUS • Lilly • Mestrelab Research •Thieme •Scharlab • Acros • ABCR • AMSLab • GalChimia • Elsevier • The Journal of the American Chemical Society • ACS Omega • Organic Letters • The Journal of Organic Chemistry • Accountsof Chemical Research. Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 15 abr 2026