RT Journal Article
T1 Genomic DNA damage induced by co-exposure to DNA damaging agents and pulsed magnetic field
A1 López-Díaz, Beatriz
A1 Mercado-Sáenz, Silvia
A1 Burgos-Molina, Antonio Manuel
A1 González Vidal, Alejandro
A1 Sendra-Portero, Francisco
A1 Ruiz-Gómez, Miguel José
K1 Acido desoxirribonucleico - Alteración
K1 Carcinógenos
K1 Campos electromagnéticos - Aspectos sanitarios
AB Purpose: Many articles describe the effects of extremely low-frequency magnetic fields (MFs) on DNA damage induction. However, the mechanism of MF interaction with living matter is not yet known with certainty. Some works suggest that MF could induce an increase in the efficacy of reactive oxygen species (ROS) production. This work investigates whether pulsed MF exposure produces alterations in genomic DNA damage induced by co-exposure to DNA damaging agents (bleomycin and methyl methanesulfonate (MMS)).Materials and methods: Genomic DNA, prepared from S. cerevisiae cultures, was exposed to pulsed MF (1.5 mT peak, 25 Hz) and MMS (0–1%) (15–60 min), and to MF and bleomycin (0–0.6 IU/mL) (24–72 h). The damage induced to DNA was evaluated by electrophoresis and image analysis. Results: Pulsed MF induced an increment in the level of DNA damage produced by MMS and bleomycin in all groups at the exposure conditions assayed.Conclusions: Pulsed MF could modulate the cytotoxic action of MMS and bleomycin. The observed effect could be the result of a multifactorial process influenced by the type of agent that damages DNA, the dose, and the duration of the exposure to the pulsed MF.
PB Taylor & Francis
YR 2023
FD 2023
LK https://hdl.handle.net/10630/39557
UL https://hdl.handle.net/10630/39557
LA eng
NO Beatriz López-Díaz, Silvia Mercado-Sáenz, Antonio M. Burgos- Molina, Alejandro González-Vidal, Francisco Sendra-Portero & Miguel J. Ruiz-Gómez (2023) Genomic DNA damage induced by co-exposure to DNA damaging agents and pulsed magnetic field, International Journal of Radiation Biology, 99:5, 853-865, DOI: 10.1080/09553002.2022.2121873
NO https://openpolicyfinder.jisc.ac.uk/id/publication/911
NO Plan Andaluz de Investigación, Desarrollo e Innovación (PAIDI); Junta de Andalucía, code CTS-181
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026